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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-Nuclear Receptor Corepressor NCoR抗体(ab3482)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (9)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

Key features and details

  • Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Nuclear Receptor Corepressor NCoR抗体
    参阅全部 Nuclear Receptor Corepressor NCoR 一抗
  • 描述

    兔多克隆抗体to核Receptor Corepressor NCoR
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Mouse Nuclear Receptor Corepressor NCoR aa 2427-2443.
    Sequence:

    PAPLLSAQYETLSDSDD


    (Peptide available as ab4997)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Nuclear Receptor Corepressor NCoR protein (ab82239)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Human
IHC-P
Mouse
Human
All applications
Rat
Xenopus laevis
Xenopus tropicalis
应用 Ab评论 说明
IHC-P (1)
1/200 - 1/1000.
ICC/IF
1/100 - 1/500.
说明
IHC-P
1/200 - 1/1000.
ICC/IF
1/100 - 1/500.

靶标

  • 功能

    Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
  • 序列相似性

    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • 结构域

    The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
    The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
  • 翻译后修饰

    Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession O75376 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9611 Human
    • Entrez Gene: 20185 Mouse
    • Entrez Gene: 54299 Rat
    • Entrez Gene: 398304 Xenopus laevis
    • Omim: 600849 Human
    • SwissProt: O75376 Human
    • SwissProt: Q60974 Mouse
    • SwissProt: Q9WUB5 Rat
    • SwissProt: Q8QG78 Xenopus laevis
    • Unigene: 462323 Human
    • Unigene: 271814 Mouse
    • Unigene: 460227 Mouse
    • Unigene: 24948 Rat
    • Unigene: 77498 Xenopus laevis
    see all
  • 别名

    • hN CoR antibody
    • hNCoR antibody
    • KIAA1047 antibody
    • N CoR antibody
    • N Cor/SMRT corepressor Rip13 antibody
    • N CoR1 antibody
    • N-CoR antibody
    • N-CoR1 antibody
    • NCOR 1 antibody
    • NCoR antibody
    • Ncor1 antibody
    • NCOR1_HUMAN antibody
    • Nuclear receptor co repressor 1 antibody
    • Nuclear receptor corepressor 1 antibody
    • Retinoid X receptor interacting protein 13 antibody
    • RIP13 antibody
    • Rxrip13 antibody
    • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
    • TRAC 1 antibody
    • TRAC1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Mouse breast tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (9)

发表研究结果有使用 ab3482?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3482 被引用在 9 文献中.

  • Jeon YG  et al. RNF20 Functions as a Transcriptional Coactivator for PPAR? by Promoting NCoR1 Degradation in Adipocytes. Diabetes 69:20-34 (2020). PubMed: 31604693
  • Zylicz JJ  et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell 176:182-197.e23 (2019). PubMed: 30595450
  • Raciti M  et al. NRXN1 Deletion and Exposure to Methylmercury Increase Astrocyte Differentiation by Different Notch-Dependent Transcriptional Mechanisms. Front Genet 10:593 (2019). PubMed: 31316548
  • Balasubramaniyan N  et al. Nuclear factor-?B regulates the expression of multiple genes encoding liver transport proteins. Am J Physiol Gastrointest Liver Physiol 310:G618-28 (2016). PubMed: 26867564
  • Kim H The transcription cofactor CRTC1 protects from aberrant hepatic lipid accumulation. Sci Rep 6:37280 (2016). WB ; Mouse . PubMed: 27869139
  • Liu YY  et al. Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation. J Biol Chem 290:7402-15 (2015). WB ; Mouse . PubMed: 25572392
  • Corbett GT  et al. Activation of peroxisome proliferator-activated receptor a stimulates ADAM10-mediated proteolysis of APP. Proc Natl Acad Sci U S A 112:8445-50 (2015). ChIP ; Mouse . PubMed: 26080426
  • Tsuji-Takechi K  et al. Stage-specific functions of leukemia/lymphoma-related factor (LRF) in the transcriptional control of osteoclast development. Proc Natl Acad Sci U S A 109:2561-6 (2012). ChIP ; Mouse . PubMed: 22308398
  • You SH  et al. 4-Hydroxy-PCB106 acts as a direct thyroid hormone receptor agonist in rat GH3 cells. Mol Cell Endocrinol 257-258:26-34 (2006). PubMed: 16930818

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 30°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Sep 22 2015

Western blot abreview for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

提交于 Feb 01 2013

Question

Hi,
I would like to know if it's possible to buy a larger Qty of NCOR1 abcam #3482 antibody. We use this antibody a lot in the laboratory and it's would be nice to have a larger Qty or a special price if we buy a couple of tubes of this antobody.

Read More

Abcam community

Verified customer

Asked on Oct 24 2012

Answer

Thank you for contacting Abcam.

We do not have any other sizes of ab3482 in stock. If you order 5 or more vials, we can offer a 5% discount and if you wish to place an order for 10 or more vials, I can out you in contact with our bulk sales team and they can work out a good deal for you.

If there is anything else I can help you with, or if you are interested in placing a bulk (>10vials) order, then please let me know.

Read More

Abcam Scientific Support

回复于 Oct 24 2012

Question

What is recommended to use a positive control for Western blotting with this antibody?

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Abcam community

Verified customer

Asked on Jul 26 2005

Answer

Thank you for your enquiry. Ab3482 was characterized in Western blotting using HeLa cell extract; by Western blot, this antibody detects a protein representing N-CoR from HeLa cell extracts. HeLa cell extract is therefore recommended as a positive control for this antibody. This antibody has so far been tested for cross-reactivity with human and has not yet been tested in other species. The datasheet previously said mouse, but I have updated it to reflect this new information that I obtained from the antibody's originator. If you have any additional questions, please let us know. We are still working on your question regarding ab84 and will get back to you soon.

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Abcam Scientific Support

回复于 Jul 27 2005

Question

I was wondering if you could tell me whether your polyclonal rabbit Ncor1 antibody binds to Ncor's DNA-binding domain or not? We plan to use it for gel-shift analysis, so it is important that it does not.

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Abcam community

Verified customer

Asked on May 24 2004

Answer

Thank you for your enquiry and your interest in our product. We have not tested this yet. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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Abcam Scientific Support

回复于 May 26 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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