Anti-Nuclear Pore O-Linked Glycoprotein抗体[RL1] (ab2734)

Submit a review Q&A (2)References (7)
Western blot - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)

Key features and details

  • Mouse monoclonal [RL1] to Nuclear Pore O-Linked Glycoprotein
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgM

概述

  • 产品名称

    Anti-Nuclear Pore O-Linked Glycoprotein抗体[RL1]

  • 描述

    小鼠单克隆抗体[RL1] to核Pore O-Linked Glycoprotein

  • 宿主

    Mouse

  • 特异性

    Detects nuclear pore-O-linked glycoprotein

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Rat, Human

  • 免疫原

    Full length protein corresponding to Rat Nuclear Pore O-Linked Glycoprotein. Pore complex-lamina fraction purified from rat liver nuclear envelopes.

  • 阳性对照

    • WB: HEK-293, THP-1, HeLa and PC-12 cell lysates. IHC-P: Rat lymph node, kidney and brain tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2734于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000.
说明
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000.

靶标

  • 相关性

    Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

  • 细胞定位

    Nuclear membrane

图片

  • Western blot - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Western blot - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    All lanes : Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution


    * an uncharacterized band at ~45 kDa. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

文献 (7)

发表研究结果有使用 ab2734？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2734 被引用在 7 文献中.

  • Gorsch LC  et al. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129:939-55 (1995). PubMed: 7744966
  • Guan T  et al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995). PubMed: 8589458
  • Byrd DA  et al. Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex. J Cell Biol 127:1515-26 (1994). PubMed: 7798308
  • Greber UF & Gerace L Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein. J Cell Biol 116:15-30 (1992). PubMed: 1370490
  • Sterne-Marr R  et al. O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import. J Cell Biol 116:271-80 (1992). PubMed: 1730755
  • Holt GD  et al. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J Cell Biol 104:1157-64 (1987). PubMed: 3571327
  • Snow CM  et al. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol 104:1143-56 (1987). PubMed: 2437126

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Question

I was just wondering if you know what nuclear pore proteins this antibody is detecting in yeast.

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Answer

Thank you for your enquiry. I can confirm that this product detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast. I hope this information will be useful to you. If you require any further information, please do not hesitate to contact me again.

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Question

Which NPC proteins does this antibody detect? I am also seeing a 250kDa band on my Western blot. Has this band been observed before?

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Answer

Thank you for your enquiry. I am unaware of any resources that mention a nuclear pore protein at 250 kDa. I did a quick literature search and did not pull up a nuclear pore protein at 250 kDa. However, I did find an interesting article that states a 250 kDa band may be another glycoprotein that is immunoprecipitated along with Nuclear pore protein 68 (pBC68). I have added the link below to where you can get a free full-text article. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_ui ds=10462521&dopt=Abstract Please do not hesitate to contact us if you need anything further.

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