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Tags & Cell Markers Subcellular Markers Nucleus Nucleoplasm

Anti-Nuclear Matrix蛋白p84抗体[5E10] (ab487)

  • Datasheet
Reviews (13) Submit a question References (72)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Immunoprecipitation - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

Key features and details

  • Mouse monoclonal [5E10] to Nuclear Matrix Protein p84
  • Suitable for: WB, ICC/IF, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

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概述

  • 产品名称

    Anti-Nuclear Matrix蛋白p84抗体[5E10]
    参阅全部 Nuclear Matrix Protein p84 一抗
  • 描述

    小鼠单克隆抗体[5E10] to核Matrix蛋白p84
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Fusion protein containing amino acids 15-374 of human p84 expressed in E. coli.

  • 阳性对照

    • WB: HEK-293T, A431, HeLa, HepG2, A-375, NIH-3T3, PC-12 whole cell lysates; HeLa nuclear lysate, mouse cerebellum, brain tissue lysates. IHC-P: Human lung cencer and breast carcinoma tissue. ICC: HeLa cells. IP: HepG2 whole cell lysate.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 2nd Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituent: 100% PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    5E10
  • 骨髓瘤

    NS1
  • 同种型

    IgG2b
  • 轻链类型

    kappa
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleoplasm
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Mouse
Human
应用 Ab评论 说明
WB (8)
Use a concentration of 0.3 - 2 µg/ml.
ICC/IF (1)
Use a concentration of 0.5 - 2 µg/ml.
IP (1)
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

说明
WB
Use a concentration of 0.3 - 2 µg/ml.
ICC/IF
Use a concentration of 0.5 - 2 µg/ml.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
  • 组织特异性

    Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
  • 序列相似性

    Contains 1 death domain.
  • 结构域

    An intact death domain is needed for apoptosis.
  • 翻译后修饰

    Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
  • 细胞定位

    Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
  • Target information above from: UniProt accession Q96FV9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9984 Human
    • Entrez Gene: 225160 Mouse
    • Entrez Gene: 291797 Rat
    • Omim: 606930 Human
    • SwissProt: Q96FV9 Human
    • SwissProt: Q8R3N6 Mouse
    • SwissProt: P59924 Rat
    • Unigene: 712543 Human
    • Unigene: 219648 Mouse
    • Unigene: 127881 Rat
    see all
  • 形式

    Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2).
  • 别名

    • hTREX84 antibody
    • Death domain containing protein p84N5 antibody
    • HPR 1 antibody
    • HPR1 antibody
    • hTREX84 antibody
    • Nuclear matrix protein p84 antibody
    • P84 antibody
    • p84N5 antibody
    • Tho 1 antibody
    • THO complex 1 antibody
    • THO complex subunit 1 antibody
    • Tho1 antibody
    • THOC 1 antibody
    • Thoc1 antibody
    • THOC1_HUMAN antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

    Immunohistochemical analysis of human lung cancer tissue labeling Nuclear Matrix Protein p84 at the nucleus with ab487 at 1/100 dilution. 

  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

    Immunocytochemical analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Nuclear Matrix Protein p84 at the nucleus with ab487 at 1/500 dilution. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

  • Immunoprecipitation - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Immunoprecipitation - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

    Nuclear Matrix Protein p84 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 3 µg ab487. Western blot was performed from the immunoprecipitate using ab487. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HepG2 whole cell lysate 30 μg.

    Lane 2: Control IP in HepG2 whole cell lysate with 3 μg of pre-immune mouse IgG.

    Lane 3: ab487 IP in HepG2 whole cell lysate.

     

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

    Overlay histogram showing HeLa cells stained with ab487 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab487, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG


    7.5% SDS-PAGE gel.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

    Immunohistochemical analysis of human breast carcinoma tissue labeling Nuclear Matrix Protein p84 at the nucleus with ab487 at 1/200 dilution. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)This image is a courtesy of an anonymous Abreview

    ab487 staining Nuclear Matrix Protein p84 in Human stomach adenocarcinoma cell line (AGS) by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.025% Triton X-100, TBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 2µg/ml for 1 hour at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 350 (blue) used undiluted.p84 shows nuclear localization.

    This image was generated using the ascites version of the product.

    See Abreview

  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
    Lane 2 : HEK-293T whole cell lysate at 10 µg
    Lane 3 : HEK-293T whole cell lysate at 5 µg

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

    ICC/IF image of ab487 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab487, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG


    7.5% SDS-PAGE gel.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
    Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution + Mouse cerebellum tissue lysates at 50 µg

    Secondary
    anti-mouse IgG HRP-conjugated antibody

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (72)

    发表研究结果有使用 ab487?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab487 被引用在 72 文献中.

    • Khamis A  et al. Histone deacetylase 9 promoter hypomethylation associated with adipocyte dysfunction is a statin-related metabolic effect. Clin Epigenetics 12:68 (2020). PubMed: 32410704
    • Xiang L  et al. FOXQ1 promotes the osteogenic differentiation of bone mesenchymal stem cells via Wnt/ß-catenin signaling by binding with ANXA2. Stem Cell Res Ther 11:403 (2020). PubMed: 32943107
    • Merthan L  et al. The role of PTB domain containing adaptor proteins on PICALM-mediated APP endocytosis and localization. Biochem J 476:2093-2109 (2019). PubMed: 31300465
    • Li G  et al. Regulator of G protein signaling 20 promotes proliferation and migration in bladder cancer via NF-?B signaling. Biomed Pharmacother 117:109112 (2019). PubMed: 31212130
    • Yang Q  et al. Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-?B pathway. J Exp Clin Cancer Res 38:263 (2019). PubMed: 31208444
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 13 Abreviews or Q&A

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (cervix)
    Loading amount
    50 µg
    Specification
    cervix
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 09 2012

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NSC34 cells)
    Loading amount
    10 µg
    Specification
    NSC34 cells
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 08 2011

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    35 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (7% Tris-Acetat)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Lukas Rambousek

    Verified customer

    提交于 Dec 22 2010

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Fibroblasts)
    Loading amount
    40 µg
    Specification
    Fibroblasts
    Gel Running Conditions
    Reduced Denaturing (4-12% BisTris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Ioannis Gavvovidis

    Verified customer

    提交于 Dec 03 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Stomach)
    Specification
    Stomach
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 27 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Stomach)
    Specification
    Stomach
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 27 2010

    Immunocytochemistry abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (AGS Gastric Carcinoma cells)
    Specification
    AGS Gastric Carcinoma cells
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 27 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (AGS)
    Specification
    AGS
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in TBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 12 2010

    Immunoprecipitation abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (AGS)
    Total protein in input
    200 µg
    Specification
    AGS
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 17 2010

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

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    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hela)
    Loading amount
    60 µg
    Specification
    Hela
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Sep 11 2009

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