Anti-Nuclear Matrix蛋白p84抗体[5E10] (ab487)

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概述

  • 产品名称
    Anti-Nuclear Matrix蛋白p84抗体[5E10]
    参阅全部 Nuclear Matrix Protein p84 一抗
  • 描述
    小鼠单克隆抗体[5E10] to核Matrix蛋白p84
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IP, ICC/IF, ICC, IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Fusion protein containing amino acids 15-374 of human p84 expressed in E. coli.

  • 阳性对照
    • Molt 4, HeLa, Raji cells. WB: HEK-293T, A431, HeLa, HepG2, A-375 whole cell lysates; HeLa nuclear lysate. ICC/IF: HeLa cells. IP: HepG2 whole cell lysate. IHC-Fr: Human stomach tissue.

性能

应用

Our Abpromise guarantee covers the use of ab487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml.
IP Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
  • 组织特异性
    Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
  • 序列相似性
    Contains 1 death domain.
  • 结构域
    An intact death domain is needed for apoptosis.
  • 翻译后修饰
    Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
  • 细胞定位
    Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
  • Information by UniProt
  • 数据库链接
    • 形式
      Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2).
    • 别名
      • hTREX84 antibody
      • Death domain containing protein p84N5 antibody
      • HPR 1 antibody
      • HPR1 antibody
      • hTREX84 antibody
      • Nuclear matrix protein p84 antibody
      • P84 antibody
      • p84N5 antibody
      • Tho 1 antibody
      • THO complex 1 antibody
      • THO complex subunit 1 antibody
      • Tho1 antibody
      • THOC 1 antibody
      • Thoc1 antibody
      • THOC1_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/500 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-mouse IgG


      7.5% SDS-PAGE gel.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

      4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nuclear Matrix Protein p84 (green) using ab487 at 1/500 dilution in ICC/IF.

      Red: phalloidin, a cytoskeleton marker, at 1/200 dilution.

    • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)This image is courtesy of an anonymous Abreview
      All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution

      Lane 1 : 0.1% DMSO treated Jurkat cell (whole cell lysate)
      Lane 2 : 0.5% DMSO treated Jurkat cell (whole cell lysate)
      Lane 3 : 1% DMSO treated Jurkat cell (whole cell lysate)
      Lane 4 : 0.1% SDS treated Jurkat cell (whole cell lysate)
      Lane 5 : 0.5% SDS treated Jurkat cell (whole cell lysate)
      Lane 6 : 1% SDS treated Jurkat cell (whole cell lysate)

      Secondary
      All lanes : HRP conjugated goat anti-mouse.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 84 kDa (why is the actual band size different from the predicted?)


      Exposure time: 1 minute


      SDS and DMSO were constituents of the lysis buffer. 0.1% SDS did not break down the nucleus entirely , however the higher concentrations did and p84 was detected in the lysate.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)This image is a courtesy of an anonymous Abreview
      ab487 staining Nuclear Matrix Protein p84 in Human stomach adenocarcinoma cell line (AGS) by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.025% Triton X-100, TBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 2µg/ml for 1 hour at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 350 (blue) used undiluted.
      p84 shows nuclear localization.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      ICC/IF image of ab487 stained Hepp cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab487, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Overlay histogram showing HeLa cells stained with ab487 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab487, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)

      Nuclear Matrix Protein p84 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 3 µg ab487. Western blot was performed from the immunoprecipitate using ab487. Anti-Rabbit IgG was used as a secondary reagent.

      Lane 1: HepG2 whole cell lysate 30 μg.

      Lane 2: Control IP in HepG2 whole cell lysate with 3 μg of pre-immune mouse IgG.

      Lane 3: ab487 IP in HepG2 whole cell lysate.

    • Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-mouse IgG


      7.5% SDS-PAGE gel.

    • Immunohistochemistry (Frozen sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)
      Immunohistochemistry (Frozen sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)This image is courtesy of an anonymous Abreview.

      Acetone-fixed frozen section of human stomach tissue stained for Nuclear Matrix Protein p84 using ab487 at 2 µg/ml in immunohistochemical analysis.

      See Abreview

    文献

    This product has been referenced in:
    • Mai HN  et al. Exposure to far-infrared rays attenuates methamphetamine-induced recognition memory impairment via modulation of the muscarinic M1 receptor, Nrf2, and PKC. Neurochem Int 116:63-76 (2018). Read more (PubMed: 29572053) »
    • Zhang Y  et al. MicroRNA-410 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells through down-regulating Wnt3a. Am J Transl Res 9:136-145 (2017). WB ; Human . Read more (PubMed: 28123640) »

    See all 47 Publications for this product

    发表研究结果有使用 ab487？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    13 reviews or Q&A

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    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (cervix)
    Loading amount
    50 µg
    Specification
    cervix
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Abcam user community

    Verified customer

    提交于 Feb 09 2012

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NSC34 cells)
    Loading amount
    10 µg
    Specification
    NSC34 cells
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    提交于 Jul 08 2011

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    35 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing (7% Tris-Acetat)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Dr. Lukas Rambousek

    Verified customer

    提交于 Dec 22 2010

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Fibroblasts)
    Loading amount
    40 µg
    Specification
    Fibroblasts
    Gel Running Conditions
    Reduced Denaturing (4-12% BisTris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Dr. Ioannis Gavvovidis

    Verified customer

    提交于 Dec 03 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Stomach)
    Specification
    Stomach
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    提交于 May 27 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Stomach)
    Specification
    Stomach
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Abcam user community

    Verified customer

    提交于 May 27 2010

    Immunocytochemistry abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (AGS Gastric Carcinoma cells)
    Specification
    AGS Gastric Carcinoma cells
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Abcam user community

    Verified customer

    提交于 May 27 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (AGS)
    Specification
    AGS
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.025% Triton-X in TBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    提交于 May 12 2010

    Immunoprecipitation abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (AGS)
    Total protein in input
    200 µg
    Specification
    AGS
    Immuno-precipitation step
    Protein A/G
    Read More
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    Abcam user community

    Verified customer

    提交于 Mar 17 2010

    Western blot abreview for Anti-Nuclear Matrix Protein p84 antibody [5E10] - Nuclear Marker

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hela)
    Loading amount
    60 µg
    Specification
    Hela
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    Username

    Abcam user community

    Verified customer

    提交于 Sep 11 2009

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