Anti-Nuclear Matrix蛋白p84抗体[5E10] (ab487)
概述
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产品名称Anti-Nuclear Matrix蛋白p84抗体[5E10]
参阅全部 Nuclear Matrix Protein p84 一抗 -
描述小鼠单克隆抗体[5E10] to核Matrix蛋白p84
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宿主Mouse
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经测试应用适用于: WB, IP, ICC/IF, ICC, IHC-P, IHC-Fr, Flow Cytmore details
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种属反应性与反应: Mouse, Human
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免疫原
Fusion protein containing amino acids 15-374 of human p84 expressed in E. coli.
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阳性对照
- Molt 4, HeLa, Raji cells. WB: HEK-293T, A431, HeLa, HepG2, A-375 whole cell lysates; HeLa nuclear lysate. ICC/IF: HeLa cells. IP: HepG2 whole cell lysate. IHC-Fr: Human stomach tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.40
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度Ascites
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纯化说明Purified from ascites.
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克隆单克隆
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克隆编号5E10
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骨髓瘤NS1
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同种型IgG2b
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轻链类型kappa
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研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
Our Abpromise guarantee covers the use of ab487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. | |
| IP | Use a concentration of 0.5 - 2 µg/ml. | |
| ICC/IF | Use a concentration of 0.5 - 2 µg/ml. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. -
组织特异性Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
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序列相似性Contains 1 death domain.
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结构域An intact death domain is needed for apoptosis.
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翻译后修饰Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
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细胞定位Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
- Information by UniProt
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数据库链接
- Entrez Gene: 9984 Human
- Entrez Gene: 225160 Mouse
- Omim: 606930 Human
- SwissProt: Q96FV9 Human
- SwissProt: Q8R3N6 Mouse
- Unigene: 712543 Human
- Unigene: 219648 Mouse
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形式Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2).
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别名
- hTREX84 antibody
- Death domain containing protein p84N5 antibody
- HPR 1 antibody
see all
图片
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![Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/ab487-291518-ab487WBa.jpg)
Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : A-375 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG7.5% SDS-PAGE gel.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/ab487-291517-ab487IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nuclear Matrix Protein p84 (green) using ab487 at 1/500 dilution in ICC/IF.
Red: phalloidin, a cytoskeleton marker, at 1/200 dilution.
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![Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/ab487_1.jpg)
Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution
Lane 1 : 0.1% DMSO treated Jurkat cell (whole cell lysate)
Lane 2 : 0.5% DMSO treated Jurkat cell (whole cell lysate)
Lane 3 : 1% DMSO treated Jurkat cell (whole cell lysate)
Lane 4 : 0.1% SDS treated Jurkat cell (whole cell lysate)
Lane 5 : 0.5% SDS treated Jurkat cell (whole cell lysate)
Lane 6 : 1% SDS treated Jurkat cell (whole cell lysate)
Secondary
All lanes : HRP conjugated goat anti-mouse.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 84 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
SDS and DMSO were constituents of the lysis buffer. 0.1% SDS did not break down the nucleus entirely , however the higher concentrations did and p84 was detected in the lysate. -
![Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/Nuclear-Matrix-Protein-p84-Primary-antibodies-ab487-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)This image is a courtesy of an anonymous Abreviewab487 staining Nuclear Matrix Protein p84 in Human stomach adenocarcinoma cell line (AGS) by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.025% Triton X-100, TBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 2µg/ml for 1 hour at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 350 (blue) used undiluted.
p84 shows nuclear localization. -
![Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/Nuclear-Matrix-Protein-p84-Primary-antibodies-ab487-8.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)ICC/IF image of ab487 stained Hepp cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab487, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/Nuclear-Matrix-Protein-p84-Primary-antibodies-ab487-13.jpg)
Flow Cytometry - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)Overlay histogram showing HeLa cells stained with ab487 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab487, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
![Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/ab487-291521-ab487IP.jpg)
Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)Nuclear Matrix Protein p84 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 3 µg ab487. Western blot was performed from the immunoprecipitate using ab487. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HepG2 whole cell lysate 30 μg.
Lane 2: Control IP in HepG2 whole cell lysate with 3 μg of pre-immune mouse IgG.
Lane 3: ab487 IP in HepG2 whole cell lysate.
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![Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/ab487-291519-ab487WBb.jpg)
Western blot - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG7.5% SDS-PAGE gel.
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![Immunohistochemistry (Frozen sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)](https://www.abcam.cn/ps/products/0/ab487/Images/ab487-291523-ab48718828.jpg)
Immunohistochemistry (Frozen sections) - Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487)This image is courtesy of an anonymous Abreview.Acetone-fixed frozen section of human stomach tissue stained for Nuclear Matrix Protein p84 using ab487 at 2 µg/ml in immunohistochemical analysis.
实验方案
数据表及文件
文献
This product has been referenced in:
- Mai HN et al. Exposure to far-infrared rays attenuates methamphetamine-induced recognition memory impairment via modulation of the muscarinic M1 receptor, Nrf2, and PKC. Neurochem Int 116:63-76 (2018). Read more (PubMed: 29572053) »
- Song J et al. Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/ß-catenin pathway in hepatocellular carcinoma. Theranostics 8:3571-3583 (2018). Read more (PubMed: 30026867) »
