Anti-Nsr1p抗体[31C4] (ab4642)

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Western blot - Anti-Nsr1p antibody [31C4] (ab4642)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)

Key features and details

  • Mouse monoclonal [31C4] to Nsr1p
  • Suitable for: ICC/IF, WB
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG

概述

  • 产品名称

    Anti-Nsr1p抗体[31C4]

  • 描述

    小鼠单克隆抗体[31C4] to Nsr1p

  • 宿主

    Mouse

  • 特异性

    This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Saccharomyces cerevisiae

  • 免疫原

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4642于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
1/500 - 1/5000. (yeast cells).
WB (1)
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.
说明
ICC/IF
1/500 - 1/5000. (yeast cells).
WB
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.

靶标

  • 相关性

    Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.

  • 细胞定位

    Nuclear

  • 数据库链接

    • 别名

      • NSR1 antibody
      • Nuclear localization sequence binding protein antibody
      • p67 antibody
      • SHE5 antibody

    图片

    • Western blot - Anti-Nsr1p antibody [31C4] (ab4642)
      Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution

      Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
      Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate

      Secondary
      All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution

      Performed under reducing conditions.

      Predicted band size: 45 kDa



      Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000)

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)
      Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      ab4642 (1/5000) detecting Nsr1p in DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
      Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
      For further experimental details please refer to Abreview.

      See Abreview

    文献 (2)

    发表研究结果有使用 ab4642？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4642 被引用在 2 文献中.

    • Azevedo C  et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018
    • Lista MJ  et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Saccharomyces cerevisiae Cell (Yeast cells DDY1810)
    Specification
    Yeast cells DDY1810
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - methanol/acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 28 2010

    Western blot abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (DDY1810)
    Loading amount
    0.5 µg
    Specification
    DDY1810
    Gel Running Conditions
    Reduced Denaturing (4-12% NUPage gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 28 2010

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