Anti-Nsr1p抗体[31C4] (ab4642)
![Western blot - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.cn/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-5.jpg "Western blot - Anti-Nsr1p antibody [31C4] (ab4642)")
Key features and details
- Mouse monoclonal [31C4] to Nsr1p
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-Nsr1p抗体[31C4] -
描述
小鼠单克隆抗体[31C4] to Nsr1p -
宿主
Mouse -
特异性
This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Sterile filtered. -
克隆
单克隆 -
克隆编号
31C4 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab4642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/500 - 1/5000. (yeast cells). | |
| WB | 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000. |
靶标
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相关性
Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 853064 Saccharomyces cerevisiae
- SwissProt: P27476 Saccharomyces cerevisiae
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别名
- NSR1 antibody
- Nuclear localization sequence binding protein antibody
- p67 antibody
- SHE5 antibody
图片
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Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution
Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate
Secondary
All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000) -
![Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)](https://www.abcam.cn/ps/products/4/ab4642/Images/Nsr1p-Primary-antibodies-ab4642-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.ab4642 (1/5000) detecting Nsr1p inDDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.
实验方案
数据表及文件
文献 (1)
ab4642 被引用在 1 文献中.
- Lista MJ et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753
