Anti-Nsr1p抗体[31C4] (ab4642)

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Key features and details

  • Mouse monoclonal [31C4] to Nsr1p
  • Suitable for: ICC/IF, WB
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG

概述

  • 产品名称

    Anti-Nsr1p抗体[31C4]

  • 描述

    小鼠单克隆抗体[31C4] to Nsr1p

  • 宿主

    Mouse

  • 特异性

    This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Saccharomyces cerevisiae

  • 免疫原

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

性能

应用

Our Abpromise guarantee covers the use of ab4642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/5000. (yeast cells).
WB 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.

靶标

  • 相关性

    Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.

  • 细胞定位

    Nuclear

  • 数据库链接

    • 别名

      • NSR1 antibody
      • Nuclear localization sequence binding protein antibody
      • p67 antibody
      • SHE5 antibody

    图片

    • Western blot - Anti-Nsr1p antibody [31C4] (ab4642)
      Western blot - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution

      Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
      Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate

      Secondary
      All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution

      Performed under reducing conditions.

      Predicted band size: 45 kDa



      Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000)

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)
      Immunocytochemistry/ Immunofluorescence - Anti-Nsr1p antibody [31C4] (ab4642)Image courtesy of an anonymous Abreview.
      ab4642 (1/5000) detecting Nsr1p in DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
      Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
      For further experimental details please refer to Abreview.

      See Abreview

    文献 (1)

    发表研究结果有使用 ab4642？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4642 被引用在 1 文献中.

    • Lista MJ  et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Saccharomyces cerevisiae Cell (Yeast cells DDY1810)
    Specification
    Yeast cells DDY1810
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - methanol/acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 28 2010

    Western blot abreview for Anti-Nsr1p antibody [31C4] - Nucleolar Marker

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (DDY1810)
    Loading amount
    0.5 µg
    Specification
    DDY1810
    Gel Running Conditions
    Reduced Denaturing (4-12% NUPage gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 28 2010

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