Key features and details
- Mouse monoclonal [31C4] to Nsr1p
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
描述小鼠单克隆抗体[31C4] to Nsr1p
特异性This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.
经测试应用适用于: ICC/IF, WBmore details
种属反应性与反应: Saccharomyces cerevisiae
This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Concentration information loading...
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab4642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/5000. (yeast cells).|
|WB||1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.|
相关性Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.
- NSR1 antibody
- Nuclear localization sequence binding protein antibody
- p67 antibody
- SHE5 antibody
All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution
Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate
All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000)
ab4642 (1/5000) detecting Nsr1p in
DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.
ab4642 被引用在 1 文献中.
- Lista MJ et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753