Key features and details
- Rabbit polyclonal to NSE - Neuronal Marker
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
产品名称Anti-NSE抗体- Neuronal Marker
参阅全部 NSE 一抗
描述兔多克隆抗体to NSE - Neuronal Marker
特异性This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. This antibody gives a specific staining in neurons of the brain, in islets of langerhans in pancreas and in peripheral nerves of the skin. No staining with other tissue types has been seen.
Tested Applications & Species
Application Species IHC-PHuman
Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
纯度Immunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
功能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
组织特异性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
序列相似性Belongs to the enolase family.
发展阶段During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
细胞定位Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
- Information by UniProt
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
ab834 被引用在 1 文献中.
- Tang YY et al. Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury. Biol Pharm Bull 40:1630-1637 (2017). PubMed: 28966235