存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab80417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
功能Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.
组织特异性Liver. Low levels of expression were detected in heart and pancreas.
序列相似性Belongs to the nuclear hormone receptor family. NR0 subfamily.
翻译后修饰Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
细胞定位Nucleus. Cytoplasm. Colocalizes with NEUROD1 in the nucleus.
- Information by UniProt
- NR0B2 antibody
- NR0B2_HUMAN antibody
- Nr0b2a antibody
Anti-NR0B2 antibody (ab80417) at 1 µg/ml + Transfected 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 20 kDa, 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab80417 has not yet been referenced specifically in any publications.