Anti-NQO1抗体(ab2346)
Key features and details
- Goat polyclonal to NQO1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
概述
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产品名称
Anti-NQO1抗体
参阅全部 NQO1 一抗 -
描述
山羊多克隆抗体to NQO1 -
宿主
Goat -
特异性
This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1) -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human, Pig
预测可用于: Dog
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免疫原
Synthetic peptide corresponding to Human NQO1 aa 267-274 (C terminal).
Sequence:C-SIPTDNQIKARK
(Peptide available asab22904) -
常规说明
Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 - 10 µg/ml.
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| WB |
Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 26-30 kDa.
Primary incubation for 1 hour at room temperature. |
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
|
WB
Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 26-30 kDa. Primary incubation for 1 hour at room temperature. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
序列相似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 610935 Dog
- Entrez Gene: 1728 Human
- Entrez Gene: 100286873 Pig
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: P05982 Rat
- Unigene: 406515 Human
see all -
别名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
图片
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Western blot - Anti-NQO1 antibody (ab2346)Anti-NQO1 antibody (ab2346) at 0.1 µg + U251 cell lysate in RIPA buffer at 35 µg
Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)Immunocytochemistry/Immunofluorescence analysis of HepG2 labelling NQO1 with ab2346 at 5 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (5 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Western blot - Anti-NQO1 antibody (ab2346)All lanes : Anti-NQO1 antibody (ab2346) at 0.03 µg
Lane 1 : Human kidney tissue lysate in RIPA buffer
Lane 2 : Human lung tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)Immunocytochemistry/Immunofluorescence analysis of U251 labelling NQO1 with ab2346 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Western blot - Anti-NQO1 antibody (ab2346)All lanes : Anti-NQO1 antibody (ab2346) at 1 µg
Lane 1 : Rat kidney tissue lysate in RIPA buffer
Lane 2 : Pig kidney tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.Detected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab2346)ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (86)
ab2346 被引用在 86 文献中.
- Hamada S et al. Nrf2 Activation Sensitizes K-Ras Mutant Pancreatic Cancer Cells to Glutaminase Inhibition. Int J Mol Sci 22:N/A (2021). PubMed: 33672789
- Weng B et al. Nrf2-Keap1-ARE-NQO1 signaling attenuates hyperoxia-induced lung cell injury by inhibiting apoptosis. Mol Med Rep 23:N/A (2021). PubMed: 33495821
- Yagishita Y et al. Constitutive Activation of Nrf2 in Mice Expands Enterogenesis in Small Intestine Through Negative Regulation of Math1. Cell Mol Gastroenterol Hepatol 11:503-524 (2021). PubMed: 32896624
- Zhang W et al. Anti-inflammatory effect of resveratrol attenuates the severity of diabetic neuropathy by activating the Nrf2 pathway. Aging (Albany NY) 13:10659-10671 (2021). PubMed: 33770763
- Nabeshima T et al. Keap1 deletion accelerates mutant K-ras/p53-driven cholangiocarcinoma. Am J Physiol Gastrointest Liver Physiol 318:G419-G427 (2020). PubMed: 31961719