重组Anti-NPM1/ALK抗体[EPR11413] (ab180607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11413] to NPM1/ALK
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NPM1/ALK抗体[EPR11413]
参阅全部 NPM1/ALK 一抗 -
描述
兔单克隆抗体[EPR11413] to NPM1/ALK -
宿主
Rabbit -
特异性
In more than 50% of systemic Anaplastic large cell lymphoma, t(2;5)(p23;q35) translocation can be found (Falini et al., 1999; Morris et al., 2001). (PMID: 9736036 and PMID: 11380391). The corresponding genes have been cloned and represent a fusion between the NPM and the ALK genes (Morris et al., 1994). (PMID: 8122112). Encodes a chimeric 80-kDa protein consisting of the N-terminal portion (amino acids 1-117) of NPM fused to the cytoplasmic portion of ALK (amino acids 1058-1620). Reference: Galietta et al., 2007. (PMID: 17537995). -
经测试应用
适用于: IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human large cell lymphoma tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11413 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180607于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. For unpurified use at 1/4000. |
说明 |
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IHC-P
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. For unpurified use at 1/4000. |
靶标
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相关性
In more than 50% of systemic Anaplastic large cell lymphoma, t(2;5)(p23;q35) translocation can be found (Falini et al., 1999; Morris et al., 2001). (PMID: 9736036 and PMID: 11380391). The corresponding genes have been cloned and represent a fusion between the NPM and the ALK genes (Morris et al., 1994). (PMID: 8122112). Encodes a chimeric 80-kDa protein consisting of the N-terminal portion (amino acids 1-117) of NPM fused to the cytoplasmic portion of ALK (amino acids 1058-1620). -
数据库链接
- SwissProt: P06748 Human
- SwissProt: Q9UM73 Human
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human anaplastic large cell lymphoma tissue sections labeling NPM1/ALK with purified ab180607 at 1/16,000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemical staining of formalin fixed paraffin-embedded Human anaplastic large cell lymphoma labeling ALK with ab180607 (unpurified) at 1/4000 dilution. HRP polymer for rabbit IgG was used as a secondary. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab180607 被引用在 3 文献中.
- Kourentzi K et al. Recombinant expression, characterization, and quantification in human cancer cell lines of the Anaplastic Large-Cell Lymphoma-characteristic NPM-ALK fusion protein. Sci Rep 10:5078 (2020). PubMed: 32193476
- Rajan SS et al. CRISPR genome editing of murine hematopoietic stem cells to create Npm1-Alk causes ALK+ lymphoma after transplantation. Blood Adv 3:1788-1794 (2019). PubMed: 31189527
- Sun Z et al. Downregulation of NPM expression by Her-2 reduces resistance of gastric cancer to oxaliplatin. Oncol Lett 13:2377-2384 (2017). IHC-P ; Human . PubMed: 28454407