Anti-NPAT抗体(ab99436)
Key features and details
- Rabbit polyclonal to NPAT
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NPAT抗体
参阅全部 NPAT 一抗 -
描述
兔多克隆抗体to NPAT -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human NPAT aa 700-800.
Database link: Q14207 -
阳性对照
- Human 293T cell line.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99436于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 4863 Human
- Omim: 601448 Human
- SwissProt: Q13632 Human
- SwissProt: Q14207 Human
- SwissProt: Q16580 Human
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别名
- E14 antibody
- nuclear protein ataxia telangiectasia locus antibody
- nuclear protein in the AT region antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (ab99436)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling NPAT with ab99436 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-NPAT antibody (ab99436)Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab99436 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in laneWestern Blot: an antibody that recognizes a downstream epitope of NPAT at 1 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 154 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab99436 尚未被引用在任何文献中。