Anti-Noxa抗体(ab36833)
Key features and details
- Rabbit polyclonal to Noxa
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Noxa抗体
参阅全部 Noxa 一抗 -
描述
兔多克隆抗体to Noxa -
宿主
Rabbit -
特异性
Although immunogen is from Mouse, customer feedback indicates that the results for Mouse are non-specific (see Abreviews). -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa. (Peptide available as ab39823.)
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阳性对照
- Human stomach tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36833于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 6 kDa.
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IHC-P |
Use a concentration of 1 µg/ml.
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 6 kDa. |
IHC-P
Use a concentration of 1 µg/ml. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. -
组织特异性
Highly expressed in adult T-cell leukemia cell line. -
序列相似性
Belongs to the PMAIP1 family. -
结构域
The BH3 motif is essential for pro-apoptotic activity. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 5366 Human
- Entrez Gene: 492821 Rat
- Omim: 604959 Human
- SwissProt: Q13794 Human
- SwissProt: Q5U777 Rat
- Unigene: 96 Human
- Unigene: 163380 Rat
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别名
- Adult T cell leukemia derived PMA responsive antibody
- APR antibody
- APR_HUMAN antibody
see all
图片
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Anti-Noxa antibody (ab36833) at 0.5 µg/ml + Human stomach cell lysate
Predicted band size: 6 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted? -
ab36833 at 1µg/ml staining Noxa in human stomach tissue by IHC
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ICC/IF image of ab36833 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36833, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunofluorescence of Noxa in Human Stomach cells using ab36833 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab36833 被引用在 16 文献中.
- Song MZ et al. (3R)-5,6,7-trihydroxy-3-isopropyl-3-methylisochroman-1-one inhibited osteosarcoma growth by inducing apoptosis. Exp Ther Med 18:1107-1114 (2019). PubMed: 31316606
- Zheng YJ et al. Silencing lncRNA LOC101928963 Inhibits Proliferation and Promotes Apoptosis in Spinal Cord Glioma Cells by Binding to PMAIP1. Mol Ther Nucleic Acids 18:485-495 (2019). PubMed: 31670198
- Cano-González A et al. Involvement of both caspase-8 and Noxa-activated pathways in endoplasmic reticulum stress-induced apoptosis in triple-negative breast tumor cells. Cell Death Dis 9:134 (2018). IF . PubMed: 29374147
- Dong Z et al. HDAC inhibitor PAC-320 induces G2/M cell cycle arrest and apoptosis in human prostate cancer. Oncotarget 9:512-523 (2018). PubMed: 29416632
- Li W et al. Repression of Noxa by Bmi1 contributes to deguelin-induced apoptosis in non-small cell lung cancer cells. J Cell Mol Med 22:6213-6227 (2018). PubMed: 30255595