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Kits/ Lysates/ Other Tools and Reagents IHC Tools/ Reagents

Normal山羊Serum (ab7481)

  • Datasheet
Reviews (2)Q&A (10)References (201)

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Functional Studies - Normal Goat Serum (ab7481)
  • Functional Studies - Normal Goat Serum (ab7481)

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概述

  • 产品名称

    Normal山羊Serum
    参阅全部 Goat Serum 试剂
  • 宿主

    Goat
  • 经测试应用

    适用于: Blocking, IHC-Fr, ICC/IF, IHC-Pmore details
  • 常规说明

    Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

    The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

    Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised. 

    Serum can be used directly for blocking, or as a constituent of a blocking buffer.

     

    Strain: Mixed breed and sex.

    Raised in: Goat

    Purity: Whole antiserum

    This product is for research use only and not intended for diagnostic or therapeutic use of any kind. 

性能

  • 形式

    Liquid
  • 存放说明

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituent: Whole serum
  • Concentration information loading...
  • Reagent说明

    Strain: Mixed breed and sex.
  • 研究领域

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Serum Products

相关产品

  • Related Products

    • Normal Goat Serum (Sterile) (ab138478)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
    • Goat Anti-Mouse IgG H&L (HRP) (ab6789)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7481于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Blocking
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent dilution.
说明
Blocking
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent dilution.

图片

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Dzinic, Sijana H et al., PloS one vol. 8,11 e74502., Fig 2, doi:10.1371/journal.pone.0074502

    Cells grown in 8-well chamber slides to 70% confluence were fixed with 4% paraformaldehyde (15 min at room temperature (RT)), and permeabilized with 100% ice cold methanol (10 min at 20°C). The slides were incubated with 10% normal goat serum (ab7481) in PBS for 1 hr, and incubated with anti-maspin (1:100) antibody alone or in a combination with either anti-lamin B (1:50), anti-HDAC1 (1:50) or anti-GRP78 (1:50) at 4°C overnight. Cells were washed and incubated for 2 hrs at room temperature (RT) with Alexa Fluor 488 (1:500) alone or in combination with Alexa Fluor 594 (1:500). The nuclei were counterstained with DAPI.
    DU145 cells infected with adenovirus expressing either maspinWT or maspinD346E Confocal immunofluorescence imaging of maspin (green) and nuclear envelope marker lamin B (red) in DU145 cells after infection.

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Witasp, Anna et al., PloS one vol. 8,5 e63493., Fig 3, doi:10.1371/journal.pone.0063493
    Slides were pretreated with Hydrogen Peroxide Block followed by 15% normal goat serum (Abcam, Cambridge, UK) for 1 hour. The primary antibody, anti-PTX3, N-terminal antibody produced in rabbit was diluted 1:300 in PBS with 2.5% goat serum (ab7481), applied to slides and incubated for 3 hours at 4°C. The primary antibody was omitted in the negative controls. The PTX3 binding was revealed using a universal secondary antibody polymer formulation conjugated to horseradish peroxidase (HRP). The HRP activity was subsequently visualized with diaminobenzidine (DAB) substrate/chromogen and counterstaining with hematoxylin was performed.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (201)

发表研究结果有使用 ab7481?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7481 被引用在 201 文献中.

  • Jin Q  et al. Allisartan isoproxil attenuates oxidative stress and inflammation through the SIRT1/Nrf2/NF-?B signalling pathway in diabetic cardiomyopathy rats. Mol Med Rep 23:N/A (2021). PubMed: 33495841
  • Meng F  et al. Naloxone Facilitates Contextual Learning and Memory in a Receptor-Independent and Tet1-Dependent Manner. Cell Mol Neurobiol 41:1031-1038 (2021). PubMed: 32989585
  • Alkhalifa H  et al. Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy. Oncol Lett 21:458 (2021). PubMed: 33907568
  • Yatsenko AS  et al. Exocyst-mediated membrane trafficking of the lissencephaly-associated ECM receptor dystroglycan is required for proper brain compartmentalization. Elife 10:N/A (2021). PubMed: 33620318
  • Yang X  et al. FOXD3-AS1/miR-128-3p/LIMK1 axis regulates cervical cancer progression. Oncol Rep 45:N/A (2021). PubMed: 33760158
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 12 Abreviews or Q&A

Pax7 cells stained with ab187339 using normal goat serum ab7481

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I've been using this normal goat serum for all of my blocking on mouse skeletal muscle frozen sections and I'm very happy with its performance.

Abcam user community

Verified customer

提交于 Oct 28 2021

Perfect for immunostain

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I've been using this product for a couple of years as a blocking agent before an immunostain of cultured cells and tissue samples.
So far so good. I'll keep using this product.

Mr. Manuel Chacon

Verified customer

提交于 Oct 04 2021

Question

I bought normal goat serum and plan to use it as a blocking reagent before an immunostain of cells and tissue. Should I heat-inactivate the serum before use?

Read More

Abcam community

Verified customer

Asked on Jan 28 2016

Answer

There is no need to heat-inactivate serum if you are using it as a blocking reagent before staining fixed cells or tissue. Heat-inactivation is only necessary if you are using the serum for tissue culture. (The heat inactivates complement, and this eliminates complement-mediated cell lysis).

Read More

Tom Ruyle

Abcam Scientific Support

回复于 Jan 28 2016

Question

I'm trying to understand the difference between ab138478and ab7481. I will be using the serum for blocking of IHC on frozen tissue with fluorescent tagged antibodies.

Read More

Abcam community

Verified customer

Asked on Dec 07 2013

Answer

ab138478 is sterilized, whereas ab7481 is not. That is the only difference between the two products and the reason that ab138478 is more expensive.

Read More

Kevin Hanson

Abcam Scientific Support

回复于 Dec 07 2013

Question

Kindly advise if ab7481 is guaranteed by Abpromise as a blocking for IP procedure.

Read More

Abcam community

Verified customer

Asked on Mar 12 2012

Answer

ab7481 was not experimentally tested as blocking agent in IP procedures. But I am confident that it should be suitable in pre-clearing steps etc in the IP procedure. Please follow this link to a protocol for IP:
https://www.abcam.com/index.html?pageconfig=resource&rid=11385

Read More

Abcam Scientific Support

回复于 Mar 12 2012

Question

On the datasheet of these sera I have a concentration of 86mg/ml for the goat serum (8,6% concentration) and 71mg/ml for the rabbit serum (7,1% concentration). So no chance I can use them at 10% for IHC blocking on free-floating sections? 

Read More

Abcam community

Verified customer

Asked on Feb 07 2012

Answer

Those values are for whole protein value which we supply as a customer service. The total protein value is not considered when making blocking solutions as the percentage used is of the whole serum. Therefore to create a 100 ml or a 10% IHC block you would use 10 ml of serum in 90 ml of diluent.

Read More

Abcam Scientific Support

回复于 Feb 07 2012

Question

What is the optimal dilution to use these sera for blocking in IHC on free-floating sections?

Read More

Abcam community

Verified customer

Asked on Feb 01 2012

Answer

According to the standard protocol we have for free floating sections, we recommend to incubate the sections with 0.1M PB/0.3% triton/1% blocking serum for at least 1 h.
The whole protocol can be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=13633

As always certain steps including the amount of blocking agent might have to be optimized according to circumstances like sample, results, etc.

Read More

Abcam Scientific Support

回复于 Feb 01 2012

Question

I would like to know if I need to heat-inactivate goat serum before aliquoting and storing, and the best protocol to do it.

Read More

Abcam community

Verified customer

Asked on Feb 01 2012

Answer

To use this serum for IHC in the blocking step, it does not have to be heat treated. The heat treatment is done to inactivate complement in FCS meant for cell culture for example.

I recommend to aliquot and freeze the serum for storage.

Read More

Abcam Scientific Support

回复于 Feb 01 2012

Question

What is the optimal dilution for ab7481?

Read More

Abcam community

Verified customer

Asked on Jan 31 2012

Answer

The dilution that works best for your experiment depends on multiple variables so you should do some titrations to find the best dilution. A good starting point for ab7481 is 10%.

Read More

Abcam Scientific Support

回复于 Jan 31 2012

Question

Was this product heat inactivated?

Read More

Abcam community

Verified customer

Asked on Jun 07 2007

Answer

ab7481 (Normal Goat Serum) is not heat-inactivated.

Read More

Abcam Scientific Support

回复于 Jun 07 2007

1-10 of 12 Abreviews or Q&A

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