Normal山羊Serum (ab7481)

Submit a review Q&A (21)References (64)

概述

  • 产品名称

    Normal山羊Serum
    参阅全部 Goat Serum 试剂

  • 宿主

    Goat

  • 经测试应用

    适用于: Blocking, IHC-Fr, ICC/IF, IHC-Pmore details

  • 常规说明

    Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

    The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

    Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised. 

    Serum can be used directly for blocking, or as a constituent of a blocking buffer.

     

    Strain: Mixed breed and sex.

    Raised in: Goat

    Purity: Whole antiserum

    This product is for research use only and not intended for diagnostic or therapeutic use of any kind. 

性能

应用

Our Abpromise guarantee covers the use of ab7481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

图片

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Dzinic, Sijana H et al., PloS one vol. 8,11 e74502., Fig 2, doi:10.1371/journal.pone.0074502

    Cells grown in 8-well chamber slides to 70% confluence were fixed with 4% paraformaldehyde (15 min at room temperature (RT)), and permeabilized with 100% ice cold methanol (10 min at 20°C). The slides were incubated with 10% normal goat serum (ab7481) in PBS for 1 hr, and incubated with anti-maspin (1:100) antibody alone or in a combination with either anti-lamin B (1:50), anti-HDAC1 (1:50) or anti-GRP78 (1:50) at 4°C overnight. Cells were washed and incubated for 2 hrs at room temperature (RT) with Alexa Fluor 488 (1:500) alone or in combination with Alexa Fluor 594 (1:500). The nuclei were counterstained with DAPI.
    DU145 cells infected with adenovirus expressing either maspinWT or maspinD346E Confocal immunofluorescence imaging of maspin (green) and nuclear envelope marker lamin B (red) in DU145 cells after infection.

  • Functional Studies - Normal Goat Serum (ab7481)
    Functional Studies - Normal Goat Serum (ab7481)Witasp, Anna et al., PloS one vol. 8,5 e63493., Fig 3, doi:10.1371/journal.pone.0063493
    Slides were pretreated with Hydrogen Peroxide Block followed by 15% normal goat serum (Abcam, Cambridge, UK) for 1 hour. The primary antibody, anti-PTX3, N-terminal antibody produced in rabbit was diluted 1:300 in PBS with 2.5% goat serum (ab7481), applied to slides and incubated for 3 hours at 4°C. The primary antibody was omitted in the negative controls. The PTX3 binding was revealed using a universal secondary antibody polymer formulation conjugated to horseradish peroxidase (HRP). The HRP activity was subsequently visualized with diaminobenzidine (DAB) substrate/chromogen and counterstaining with hematoxylin was performed.

文献

This product has been referenced in:

  • Guo Y  et al. Carnosine improves diabetic retinopathy via the MAPK/ERK pathway. Exp Ther Med 17:2641-2647 (2019). Read more (PubMed: 30930967) »
  • Xu J  et al. LYPD8 regulates the proliferation and migration of colorectal cancer cells through inhibiting the secretion of IL-6 and TNF-a. Oncol Rep 41:2389-2395 (2019). Read more (PubMed: 30816524) »
See all 64 Publications for this product

发表研究结果有使用 ab7481？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1-10 of 21 Abreviews or Q&A

Question

I bought normal goat serum and plan to use it as a blocking reagent before an immunostain of cells and tissue. Should I heat-inactivate the serum before use?

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Answer

There is no need to heat-inactivate serum if you are using it as a blocking reagent before staining fixed cells or tissue. Heat-inactivation is only necessary if you are using the serum for tissue culture. (The heat inactivates complement, and this eliminates complement-mediated cell lysis).

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Question

I'm trying to understand the difference between two products. ab138478 - sterile goat serum - 50ml - $260 and ab7481 - sterile goat serum - 50ml - $175 I will be using the serum for blocking of IHC on frozen tissue with fluorescent tagged antibodies

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Answer

ab138478 is sterilized, whereas ab7481 is not. That is the only difference between the two products and the reason that ab138478 is more expensive.

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Question

 I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards                                                    

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Answer

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choice will be personal preference of host. These products are below. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab16284: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab97051: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below. DAB substrate kit:https://www.abcam.com/DAB-substrate-kit-ab94665.html Donkey serum:https://www.abcam.com/Donkey-Serum-ab7475.html Goat serum:https://www.abcam.com/Goat-Serum-ab7481.html Antigen retrieval buffer:https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Question

I have one question for you. My customer wants to buy serum "goat" and "donkey" for blocking nonspecific effects in imunohistochemistry. Do you have something like this in your offer?

Thank you for your answer.

Best regards

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Answer

Thank you for contacting us.

The goat serum is ab7481; https://www.abcam.com/Goat-Serum-ab7481.html

Donkey serum ab7475; https://www.abcam.com/Donkey-Serum-ab7475.html

ab138478; https://www.abcam.com/Goat-Serum-sterile-ab138478.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I am using your HIF-1 alpha HIF1 alpha (cat 16066) : lot no 906012 for human gliomas on FFPE tissue for IHC.

In order to optimise my results, I have been using goat serum for diluting the antibody. (protocol attached)

I had to procure from some other source.

Now after completing my initial project successfully, I want to use the remaining antibody for another project.

However, I have run out of goat serum.

May I request you to kindly arrange 5-10 ml goat serum for diluting the antibody for further use, please?

Hope to get an early response.

Regards,

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Answer

Thank you for contacting us.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products. Therefore I am sorry that I cannot answer positively to your request.

Please do not hesitate to contact us or our local distributor with any other questions.

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Question

It is not idea of searching the items.. I told you that we need all reagents needed to do full IHC experiment based on your technical experience.. So if we need more items other than the ones who sent to me, please let me know.. Like antibosy diluent, antigen retrival .. etc, The customers is new to IHC and we are not experts in IHC as well.. So please advice..



It is ok about the monoclonal rabies, waiting your kind e-mail !



Truly yours,

Omar

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Answer

Thank you for contacting us again.



ab1002 Anti-Rabies Virus antibody looks very promising as it has been established for the use in IHC-P and should detect a wide range of rabies strains. As a starting dilution the customer can try a 5 ug/ml dilution incubated overnight at 4C. However, it may be that the actual optimum dilution may be lower or higher, but this depends on her samples. Another point is that I would strongly recommend a heat induced Antigen Retrieval if her slides are fixed with formalin.


https://www.abcam.com/index.html?datasheet=1002 (or use the following: https://www.abcam.com/index.html?datasheet=1002).


A matching secondary antibody would be Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (Biotin), pre-adsorbed (ab98696)


https://www.abcam.com/index.html?datasheet=98696 (or use the following: https://www.abcam.com/index.html?datasheet=98696).


To be fair, I am not the biggest fan of ready- to- use solution (andmost of my colleagues here a neither), for the following reason: most of all antibodies need optimization, may it be the blocking step, the retrieval time or method, the antibody incubation buffer etc. With ready-mix solutions it almost impossible to adjust the experiment settings. Ready mix solution make sense if the antibody is used in a high -throughput lab (like 20 slides at once with a fixed protocol), but not if you have to "learn" IHC-P and have the freedom to adjust the protocol fore the perfect results. In addition, it's cheaper ;)


Of course, once the protocol is optimized, using ready mixed solution are a very time efficient and make life easier, but I wouldn't use them as a beginner.


All the buffers your customer needs (see attachment) are listed in our very detailed and easy to follow step-by-step our protocol. However, none of my advice is written in stone, so if your customer prefers the ready mix solutions, we are more than happy to sell them to her.


Something we cannot provide are the reagents for the de-paraffinization, I am afraid, but a company like Sigma should be able to help with that.


Pease sent the customer our protocol and my advice. I am more than happy to assist her to get her experiments running. And another thing: she shouldn't be shy to ask any question she may have about the protocol!

I hope this more information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Product code: 7481

Inquiry: hi, we are looking for Goat Serum (ab7481) . can you please send us a quote for 50 ml of the same at the earliest please. regards,

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Answer

Thank you for your enquiry.
I can confirm that from India, Abcam products can be purchased through our Indian distributors. They will be able to provide you with a price for this product:

Central and South India:

Allied Scientific Products
39/19B Gopal Nagar Road
Kolkata-700027
India
Website http://www.alliedscientific.net
Freephone 1800 345 7777
Fax +91 33 24566683 / 24790626
Tel +91 33 24566684/24793109/9039

North India:

NuLife Consultants & Distributors (Pvt.) Ltd.
M-52, First Floor,
Lajpat Nagar- Part-II,
New Delhi-110024-India
Tel 011-46112691/ 46112692/ 29836141
Website http://www.nulife.in
Fax 91 - 011-46586216
Email nulife@bol.net.in
mailto:sales@nulife.in

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early response requested.



Sincere regards,

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Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=1012 (or use the following: https://www.abcam.com/index.html?datasheet=1012).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=12209 (or use the following: https://www.abcam.com/index.html?datasheet=12209).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=6002 (or use the following: https://www.abcam.com/index.html?datasheet=6002).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/index.html?datasheet=124462 (or use the following: https://www.abcam.com/index.html?datasheet=124462).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

ab7481 (Goat Serum)

https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

ab73430 (DNase I protein (Active))

https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

ab64220 (Proteinase K)

https://www.abcam.com/index.html?datasheet=64220 (or use the following: https://www.abcam.com/index.html?datasheet=64220).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/index.html?datasheet=65621 (or use the following: https://www.abcam.com/index.html?datasheet=65621).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Question

Thank you very much! In this way I should be able to image the aggrecan in green and the collagen II in red, is this correct? Is there any kind of discount or special price for this package?

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Answer

It is my pleasure to help.

In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttps://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those colors.

We do offer discounts on purchases of five items or more and offer a number of promotions. Our customer service representatives will be able to give you more detailed information about our current promotions and discounts or you may follow the link here:https://www.abcam.com/index.html?pageconfig=resource&rid=14853

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Question

Hello, I need to image collagen type II and aggrecan from mouse within the same samples. The laser that I have available are 458, 488, 514, 543, 633. Which combination of antibodies (primary and secondary) do you recommend that might work nicely together? Many thanks for your assistance.

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Answer

Thank you for contacting us.

We presently have only 1 product against mouse aggrecan, ab36861. This is a rabbit polyclonal. In ICC and appropriate secondary would be anti-Rabbit IgG with a fluorescent tag which could be excited by your lasers. A product such as ab96899,Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488),https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96899.html

We currently have 20 products against collagen II. However taking into account that this will have to be used in mouse tissue in conjunction with an anti-aggrecan rabbit polyclonal we can limit this to ab3092Anti-Collagen II antibody [5B2.5]. This is a mouse monoclonal antibody of isotype IgG2a that has been tested inIHC-Fr,IHC-P,WB and IF. An appropriate secondary should preferably be a F(ab) fragment as mouse on mouse staining can be difficult, please see this link for more information about mouse on mouse staining,https://www.abcam.com/index.html?pageconfig=resource&rid=11465. One such product would be ourGoat F(ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2 (DyLight® 550), pre-adsorbed,https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fab-2-DyLight-550-pre-adsorbed-ab98758.html

Abcam also offers a number of ICC and IHC reagents. As both of the secondary antibodies listed above are raised in goat, we recommend using a 5-10% Goat serum as your blocking reagent and antibody incubation solution, ab7481https://www.abcam.com/Goat-Serum-ab7481.html. We, also offer buffers and antigen retrieval solutions,https://www.abcam.com/index.html?pageconfig=resource&rid=12854, as well as mounting media for fluorescent staining ab103748,https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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