Normal驴Serum (ab7475)
概述
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产品名称
Normal驴Serum
参阅全部 Donkey Serum 试剂 -
宿主
Donkey -
经测试应用
适用于: IHC (PFA fixed), Blocking, IHC-P, IHC-Fr, ICC/IFmore details -
种属反应性
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常规说明
Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.
Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.
Serum can be used directly for blocking, or as a constituent of a blocking buffer.
Strain: Mixed breed and sex.
Raised in: Donkey
Purity: Whole antiserum
性能
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形式
Liquid -
存放说明
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
研究领域
相关产品
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Related Products
- Normal Donkey serum (sterile) (ab138579)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
- Donkey Anti-Goat IgG H&L (HRP) (ab97110)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC (PFA fixed) |
Use at an assay dependent dilution.
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| Blocking |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.
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| 说明 |
|---|
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IHC (PFA fixed)
Use at an assay dependent dilution. |
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Blocking
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution. |
图片
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Functional Studies - Normal Donkey Serum (ab7475)Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285
The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.
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Functional Studies - Normal Donkey Serum (ab7475)Krzy?anowska, Weronika et al., PloS one?vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (103)
ab7475 被引用在 103 文献中.
- Cao J et al. Identification of Microbiota within Aß Plaque in APP/PS1 Transgenic Mouse. J Mol Neurosci 71:953-962 (2021). PubMed: 33098544
- Spijkers XM et al. A directional 3D neurite outgrowth model for studying motor axon biology and disease. Sci Rep 11:2080 (2021). PubMed: 33483540
- Tombor LS et al. Single cell sequencing reveals endothelial plasticity with transient mesenchymal activation after myocardial infarction. Nat Commun 12:681 (2021). PubMed: 33514719
- Ito J et al. Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice. Elife 10:N/A (2021). PubMed: 33526170
- González-Calvo I et al. Sushi domain-containing protein 4 controls synaptic plasticity and motor learning. Elife 10:N/A (2021). PubMed: 33661101