为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Kits/ Lysates/ Other Tools and Reagents Serum Products

Normal驴Serum (ab7475)

  • Datasheet
Submit a review Q&A (5)References (103)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Functional Studies - Normal Donkey Serum (ab7475)
  • Functional Studies - Normal Donkey Serum (ab7475)

你可能会感兴趣

一抗
Product image
Anti-USP51 antibody (ab121147)
蛋白
Native Cow Bovine Serum Albumin protein (ab64009)
封闭
Product image
Normal Goat Serum (ab7481)

查看更多相关产品

概述

  • 产品名称

    Normal驴Serum
    参阅全部 Donkey Serum 试剂
  • 宿主

    Donkey
  • 经测试应用

    适用于: IHC (PFA fixed), Blocking, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性

  • 常规说明

    Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

    The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

    Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised. 

    Serum can be used directly for blocking, or as a constituent of a blocking buffer.

     

     

    Strain: Mixed breed and sex.

    Raised in: Donkey

    Purity: Whole antiserum

性能

  • 形式

    Liquid
  • 存放说明

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituent: Whole serum
  • Concentration information loading...
  • 研究领域

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Serum Products

相关产品

  • Related Products

    • Normal Donkey serum (sterile) (ab138579)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    • Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab97110)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7475于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC (PFA fixed)
Use at an assay dependent dilution.
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.
说明
IHC (PFA fixed)
Use at an assay dependent dilution.
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.

图片

  • Functional Studies - Normal Donkey Serum (ab7475)
    Functional Studies - Normal Donkey Serum (ab7475)Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285

    The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2  in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.

  • Functional Studies - Normal Donkey Serum (ab7475)
    Functional Studies - Normal Donkey Serum (ab7475)Krzy?anowska, Weronika et al., PloS one?vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243
    Anti-xCT rabbit (ab37185 Abcam) dilution: 1:200 Donkey anti-rabbit TR (ab6800 Abcam) dilution: 1:1000 Donkey normal serum (ab7475 Abcam) dilution: 10%

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (103)

发表研究结果有使用 ab7475?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7475 被引用在 103 文献中.

  • Cao J  et al. Identification of Microbiota within Aß Plaque in APP/PS1 Transgenic Mouse. J Mol Neurosci 71:953-962 (2021). PubMed: 33098544
  • Spijkers XM  et al. A directional 3D neurite outgrowth model for studying motor axon biology and disease. Sci Rep 11:2080 (2021). PubMed: 33483540
  • Tombor LS  et al. Single cell sequencing reveals endothelial plasticity with transient mesenchymal activation after myocardial infarction. Nat Commun 12:681 (2021). PubMed: 33514719
  • Ito J  et al. Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice. Elife 10:N/A (2021). PubMed: 33526170
  • González-Calvo I  et al. Sushi domain-containing protein 4 controls synaptic plasticity and motor learning. Elife 10:N/A (2021). PubMed: 33661101
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-5 of 5 Abreviews or Q&A

Question

Could you be so kind and advise me the fluorescent marked secondary antibodies for ab109632 and ab421?

Thank you.

Best regards

Read More

Abcam community

Verified customer

Asked on Dec 05 2012

Answer

Thank you for contacting us.

Please try ab97099 and ab6717.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

回复于 Dec 05 2012

Question

 I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards                                                    

Read More

Abcam community

Verified customer

Asked on Oct 11 2012

Answer

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choice will be personal preference of host. These products are below. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab16284: https://www.abcam.com/Rabbit-IgG-secondary-antibody-ab16284.html Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Ab97051: https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab97051.html Abcam also provides a number of IHC reagents and kits such as DAB substrate kits, donkey or goat sera and antigen retrieval buffers. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. I have provided links to a few of these products below. DAB substrate kit:https://www.abcam.com/DAB-substrate-kit-ab94665.html Donkey serum:https://www.abcam.com/Donkey-Serum-ab7475.html Goat serum:https://www.abcam.com/Goat-Serum-ab7481.html Antigen retrieval buffer:https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-9-0-ab93684.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Oct 11 2012

Question

I have one question for you. My customer wants to buy serum "goat" and "donkey" for blocking nonspecific effects in imunohistochemistry. Do you have something like this in your offer?

Thank you for your answer.

Best regards

Read More

Abcam community

Verified customer

Asked on Sep 19 2012

Answer

Thank you for contacting us.

The goat serum is ab7481; https://www.abcam.com/Goat-Serum-ab7481.html

Donkey serum ab7475; https://www.abcam.com/Donkey-Serum-ab7475.html

ab138478; https://www.abcam.com/Goat-Serum-sterile-ab138478.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Sep 19 2012

Question

J’aimerais savoir si vous avez les certificat d’analyse des produits suivants:
Rabbit serum ref: ab7487 lot 833644
Donkey serum ref: ab7475 lot: 854038
Sheep serum ref: ab7489 lot: GR66413-1
Merci d’avance pour votre réponse

Read More

Abcam community

Verified customer

Asked on Apr 16 2012

Answer



Veuillez trouver ci-joints les certificats d'analyse pour les produits :

Rabbit serum ref : ab7487 lot 833644
Donkey serum ref: ab7475 lot: 854038
Sheep serum ref: ab7489 lot: GR66413-1

Read More

Abcam Scientific Support

回复于 Apr 16 2012

Question

Should ab7475, donkey serum, and the other species sera be appropriate for FACS blocking as well?

Read More

Abcam community

Verified customer

Asked on Feb 21 2007

Answer

Thank you for your enquiry. ab7475 has not been tested in FACS, but the use of this product in flow cytometry/blocking step should be okay. This should be true for the other serum products - please check the datasheets. Please don't hesitate to contact us again if you have further questions.

Read More

Abcam Scientific Support

回复于 Feb 21 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2022 艾博抗(上海)贸易有限公司版权所有