Key features and details
- Rabbit polyclonal to NOL3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
参阅全部 NOL3 一抗
Tested Applications & Species
Application Species IHC-PHuman WBHuman
- HeLa whole cell lysate
This product was previously labelled as Apoptosis repressor with CARD
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Isoform 1 may be involved in RNA splicing.
Isoform 2 may inhibit apoptosis.
组织特异性Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
序列相似性Contains 1 CARD domain.
细胞定位Cytoplasm and Nucleus > nucleolus.
- Information by UniProt
- Apoptosis repressor with CARD antibody
- ARC antibody
- Muscle enriched cytoplasmic protein antibody
All lanes : Anti-NOL3 antibody (ab2002) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : KB whole cell lysate
Lane 3 : A549 whole cell lysate
Observed band size: 25 kDa why is the actual band size different from the predicted?
Immunohistochemistry of NOL3 in human heart tissue with Anti-NOL3 (ab2002) at 5 µg/ml.
Immunohistochemistry of NOL3 in skeletal muscle with Anti-NOL3 (ab2002) at 5 µg/ml.
ab2002 被引用在 1 文献中.
- Razorenova OV et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . PubMed: 24344197