Anti-Nociceptin抗体(ab216413)
Key features and details
- Rabbit polyclonal to Nociceptin
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Nociceptin抗体
参阅全部 Nociceptin 一抗 -
描述
兔多克隆抗体to Nociceptin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Cow, Human, Pig -
免疫原
Synthetic peptide corresponding to Human Nociceptin aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q13519 -
阳性对照
- Rat brain tissue. Rat brain lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab216413于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500.
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WB |
1/100 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
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说明 |
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IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa). |
靶标
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功能
Nociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.
Nocistatin: Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.
Orphanin FQ2: Has potent analgesic activity. -
组织特异性
Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation. -
序列相似性
Belongs to the opioid neuropeptide precursor family. -
翻译后修饰
Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 281414 Cow
- Entrez Gene: 5368 Human
- Entrez Gene: 18155 Mouse
- Entrez Gene: 25516 Rat
- Omim: 601459 Human
- SwissProt: O62647 Cow
- SwissProt: Q13519 Human
- SwissProt: Q64387 Mouse
see all -
别名
- N/OFQ antibody
- N23K antibody
- N23K/N27K antibody
see all
图片
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All lanes : Anti-Nociceptin antibody (ab216413) at 1/200 dilution
Lane 1 : rat brain lysate
Lane 2 : rat liver lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa15% gel.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Nociceptin with ab216413 at 1/300 dilution, followed by conjugation to the secondary antibody and DAB staining.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab216413 被引用在 2 文献中.
- Yan X et al. N/OFQ modulates orofacial pain induced by tooth movement through CGRP-dependent pathways. BMC Neurosci 22:25 (2021). PubMed: 33836649
- Guo Y et al. Neuropeptide changes in an improved migraine model with repeat stimulations. Transl Neurosci 12:523-532 (2021). PubMed: 34963819