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Neuroscience Neurotransmission Nitric Oxide NOS

Anti-nNOS (neuronal)抗体(ab5586)

  • Datasheet
Reviews (1) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
  • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab5586)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)
  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)

Key features and details

  • Rabbit polyclonal to nNOS (neuronal)
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-nNOS (neuronal)抗体
    参阅全部 nNOS (neuronal) 一抗
  • 描述

    兔多克隆抗体to nNOS (neuronal)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human nNOS (neuronal) aa 1411-1425.
    Sequence:

    NRLRSESIAFIEESK


    (Peptide available as ab5891)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Assay kits

    • Nitric Oxide Synthase Activity Assay Kit (Colorimetric) (ab211083)
    • Intracellular Nitric Oxide Synthase Detection Assay Kit (ab211085)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human nNOS (neuronal) protein (ab159005)

应用

Our Abpromise guarantee covers the use of ab5586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/500.
WB 1/100 - 1/1000. Predicted molecular weight: 160 kDa.
IHC-P 1/20 - 1/200.

靶标

  • 功能

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 组织特异性

    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • 序列相似性

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域

    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • 翻译后修饰

    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • 细胞定位

    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Target information above from: UniProt accession P29475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4842 Human
    • Entrez Gene: 18125 Mouse
    • Entrez Gene: 100009243 Rabbit
    • Entrez Gene: 24598 Rat
    • Entrez Gene: 60658 Zebrafish
    • Omim: 163731 Human
    • SwissProt: P29475 Human
    • SwissProt: Q9Z0J4 Mouse
    • SwissProt: O19132 Rabbit
    • SwissProt: P29476 Rat
    • Unigene: 654410 Human
    • Unigene: 684465 Human
    • Unigene: 684466 Human
    • Unigene: 684467 Human
    • Unigene: 442195 Mouse
    • Unigene: 44249 Mouse
    • Unigene: 10573 Rat
    see all
  • 别名

    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)

    ab5586 labelling nNOS (neuronal) in the cytoplasm of Mouse heart tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab5586)
    Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab5586)

    Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (green) showing staining in the cytoplasm and membrane of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5586 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)

    ab5586 labelling nNOS (neuronal) in the cytoplasm of Human testis tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab5586)

    ab5586 labelling nNOS (neuronal) in the cytoplasm of Human skeletal muscle (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Anti-nNOS (neuronal) antibody (ab5586) at 1/500 dilution + Mouse brain cell lysate at 25 µg

    Predicted band size: 160 kDa
    Observed band size: 160 kDa

  • Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Western blot - Anti-nNOS (neuronal) antibody (ab5586)
    Anti-nNOS (neuronal) antibody (ab5586) at 1/200 dilution + Rat brain cell lysate at 25 µg

    Predicted band size: 160 kDa
    Observed band size: 160 kDa

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (6)

    发表研究结果有使用 ab5586?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5586 被引用在 6 文献中.

    • Buniatian GH  et al. Antifibrotic Effects of Amyloid-Beta and Its Loss in Cirrhotic Liver. Cells 9:N/A (2020). PubMed: 32089540
    • Kalinichenko SG  et al. Gaseous transmitters in human retinogenesis. Acta Histochem 121:604-610 (2019). PubMed: 31113654
    • Si Z  et al. Effects of thrombolysis within 6 hours on acute cerebral infarction in an improved rat embolic middle cerebral artery occlusion model for ischaemic stroke. J Cell Mol Med 23:2468-2474 (2019). PubMed: 30697923
    • Tan C  et al. Extensive protein S-nitrosylation associated with human pancreatic ductal adenocarcinoma pathogenesis. Cell Death Dis 10:914 (2019). PubMed: 31801946
    • Xiyuan Z  et al. NO-sGC Pathway Modulates Ca2+Release and Muscle Contraction in Zebrafish Skeletal Muscle. Front Physiol 8:607 (2017). PubMed: 28878687
    • Rosa SC  et al. Nitric oxide synthase isoforms and NF-kappaB activity in normal and osteoarthritic human chondrocytes: regulation by inducible nitric oxide. Nitric Oxide 19:276-83 (2008). WB ; Human . PubMed: 18675932

    客户评价及客户问答

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-nNOS (neuronal) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Dr. Faye Doherty

    Verified customer

    提交于 Aug 17 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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