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Neuroscience Neurotransmission Nitric Oxide NOS

Anti-nNOS (neuronal)抗体(ab1376)

  • Datasheet
  • SDS
Reviews (21)Q&A (6)References (46)

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Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab1376)
  • Flow Cytometry - Anti-nNOS (neuronal) antibody (ab1376)

Key features and details

  • Goat polyclonal to nNOS (neuronal)
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human nNOS (neuronal) protein (ab159005)

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概述

  • 产品名称

    Anti-nNOS (neuronal)抗体
    参阅全部 nNOS (neuronal) 一抗
  • 描述

    山羊多克隆抗体to nNOS (neuronal)
  • 宿主

    Goat
  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性

    与反应: Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • ICC/IF: U2OS cells Flow Cytometry: KELLY cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Assay kits

    • Nitric Oxide Synthase Activity Assay Kit (Colorimetric) (ab211083)
    • Intracellular Nitric Oxide Synthase Detection Assay Kit (ab211085)
  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
  • Recombinant Protein

    • Recombinant Human nNOS (neuronal) protein (ab159005)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1376于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
说明
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 组织特异性

    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • 序列相似性

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域

    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • 翻译后修饰

    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • 细胞定位

    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Target information above from: UniProt accession P29475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 477498 Dog
    • Entrez Gene: 4842 Human
    • Omim: 163731 Human
    • SwissProt: P29475 Human
    • Unigene: 654410 Human
    • Unigene: 684465 Human
    • Unigene: 684466 Human
    • Unigene: 684467 Human
    • 别名

      • 2310005C01Rik antibody
      • BNOS antibody
      • Constitutive NOS antibody
      • EC 1.14.13.39 antibody
      • IHPS 1 antibody
      • IHPS1 antibody
      • N-NOS antibody
      • NC-NOS antibody
      • neuronal Nitric Oxide Synthase antibody
      • Neuronal NOS antibody
      • Nitric oxide synthase , neuronal, included antibody
      • Nitric oxide synthase 1 (neuronal) antibody
      • Nitric oxide synthase 1 antibody
      • Nitric oxide synthase, brain antibody
      • Nitric oxide synthase, penile neuronal, included antibody
      • NNOS antibody
      • NO antibody
      • NOS 1 antibody
      • NOS antibody
      • NOS type I antibody
      • NOS-I antibody
      • NOS1 antibody
      • NOS1_HUMAN antibody
      • Peptidyl-cysteine S-nitrosylase NOS1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab1376)
      Immunocytochemistry/ Immunofluorescence - Anti-nNOS (neuronal) antibody (ab1376)

      Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Incuabted with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).

      Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (4 µg/ml).

    • Flow Cytometry - Anti-nNOS (neuronal) antibody (ab1376)
      Flow Cytometry - Anti-nNOS (neuronal) antibody (ab1376)

      Flow cytometric analysis of paraformaldehyde fixed KELLY (Human neuroblastoma cell line) cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).

      IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (46)

    发表研究结果有使用 ab1376?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1376 被引用在 46 文献中.

    • Moiseev KY  et al. Changes of nNOS expression in the tuberal hypothalamic nuclei during ageing. Nitric Oxide 100-101:1-6 (2020). PubMed: 32283261
    • Lee L  et al. Key Aspects of Neurovascular Control Mediated by Specific Populations of Inhibitory Cortical Interneurons. Cereb Cortex 30:2452-2464 (2020). PubMed: 31746324
    • Lai NY  et al. Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense. Cell 180:33-49.e22 (2020). PubMed: 31813624
    • Yang W  et al. Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model. Cell Prolif 53:e12756 (2020). PubMed: 31943490
    • Bocchio M  et al. Hippocampal hub neurons maintain distinct connectivity throughout their lifetime. Nat Commun 11:4559 (2020). PubMed: 32917906
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 27 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-nNOS (neuronal) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Proximal colon)
    Specification
    Proximal colon
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 15 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-nNOS (neuronal) antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord, MPG)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH6.0
    Permeabilization
    No
    Specification
    Spinal cord, MPG
    Blocking step
    Protein Block Serum-Free from Dako® as blocking agent for 30 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    淳太朗 小山

    Verified customer

    提交于 Oct 23 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-nNOS (neuronal) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate PH 6,6
    Permeabilization
    Yes - Triton X100 0.5%
    Specification
    stomach
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Julien Chevalier

    Verified customer

    提交于 Aug 08 2016

    IHC - Wholemount abreview for Anti-nNOS (neuronal) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Pig Tissue (Intestinal myenteric plexus)
    Specification
    Intestinal myenteric plexus
    Read More

    Mr. Julien Chevalier

    Verified customer

    提交于 Aug 08 2016

    IHC - Wholemount abreview for Anti-nNOS (neuronal) antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Tissue (Intestinal myenteric plexus)
    Specification
    Intestinal myenteric plexus
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 08 2016

    IHC - Wholemount abreview for Anti-nNOS (neuronal) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Tissue (Intestinal myenteric plexus)
    Specification
    Intestinal myenteric plexus
    Read More

    Mr. Julien Chevalier

    Verified customer

    提交于 Aug 08 2016

    IHC - Wholemount abreview for Anti-nNOS (neuronal) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Intestinal myenteric plexus)
    Specification
    Intestinal myenteric plexus
    Read More

    Mr. Julien Chevalier

    Verified customer

    提交于 Aug 08 2016

    Western blot abreview for Anti-nNOS (neuronal) antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 18 2016

    Western blot abreview for Anti-nNOS (neuronal) antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 11 2016

    Western blot abreview for Anti-nNOS (neuronal) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 08 2016

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