Anti-nNOS (neuronal)抗体(ab1376)
Key features and details
- Goat polyclonal to nNOS (neuronal)
- Suitable for: IHC-Fr, IHC-P, IHC-FoFr, WB, ICC, Flow Cyt (Intra), IHC-FrFl
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-nNOS (neuronal)抗体
参阅全部 nNOS (neuronal) 一抗 -
描述
山羊多克隆抗体to nNOS (neuronal) -
宿主
Goat -
经测试应用
适用于: IHC-Fr, IHC-P, IHC-FoFr, WB, ICC, Flow Cyt (Intra), IHC-FrFlmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog
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免疫原
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
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阳性对照
- ICC: HeLa cells. Flow Cyt (intra): KELLY cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration. PubMed: 22589245
|
| IHC-P | (9) |
Use at an assay dependent concentration. PubMed: 16263087
|
| IHC-FoFr |
Use at an assay dependent concentration.
Ovenight incubation at room temp. |
|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 160 kDa.
Lyse in RIPA buffer. |
| ICC |
Use a concentration of 10 µg/ml.
|
|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
| IHC-FrFl | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. PubMed: 22589245 |
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IHC-P
Use at an assay dependent concentration. PubMed: 16263087 |
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IHC-FoFr
Use at an assay dependent concentration. Ovenight incubation at room temp. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 160 kDa. Lyse in RIPA buffer. |
|
ICC
Use a concentration of 10 µg/ml. |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
IHC-FrFl
Use at an assay dependent concentration. |
靶标
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功能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
组织特异性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
结构域
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻译后修饰
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
细胞定位
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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数据库链接
- Entrez Gene: 477498 Dog
- Entrez Gene: 4842 Human
- Omim: 163731 Human
- SwissProt: P29475 Human
- Unigene: 654410 Human
- Unigene: 684465 Human
- Unigene: 684466 Human
- Unigene: 684467 Human
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别名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
图片
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Immunocytochemistry - Anti-nNOS (neuronal) antibody (ab1376)
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Flow Cytometry (Intracellular) - Anti-nNOS (neuronal) antibody (ab1376)Flow cytometric analysis of paraformaldehyde fixed KELLY (Human neuroblastoma cell line) cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
数据表及文件
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Datasheet download
文献 (60)
ab1376 被引用在 60 文献中.
- Özdemir-Kumral ZN et al. High-fat Diet Enhances Gastric Contractility, but Abolishes Nesfatin-1-induced Inhibition of Gastric Emptying. J Neurogastroenterol Motil 27:265-278 (2021). PubMed: 33795544
- Abot A et al. Identification of new enterosynes using prebiotics: roles of bioactive lipids and mu-opioid receptor signalling in humans and mice. Gut 70:1078-1087 (2021). PubMed: 33020209
- Yilmaz O et al. An Immunohistochemical Study on the Presence of Nitric Oxide Synthase Isoforms (nNOS, iNOS, eNOS) in the Spinal Cord and Nodose Ganglion of Rats Receiving Ionising Gamma Radiation to their Liver. J Vet Res 64:445-453 (2020). PubMed: 32984637
- Kakizawa S et al. Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells. Sci Rep 10:14475 (2020). PubMed: 32879382
- Masugi-Tokita M et al. Metabotropic Glutamate Receptor Subtype 7 Is Essential for Ejaculation. Mol Neurobiol 57:5208-5218 (2020). PubMed: 32865662
