Anti-nNOS (neuronal)抗体(ab1376)

Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded marmoset cerebellum tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

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ab1376 nNos antibody immunostaining nNos in dorsal root ganglion (DRG) neurons of an animal who had a spinal nerve ligation 2 weeks previous. [A] Image taken X10 objective, [B]Image taken with X20 objective; the staining is restricted to a subpopulation of neurons and is localized in the cytoplasm of these cells; Scale bar=20 microns. ab1376 was used at 1/300 overnight incubation at room temperature on 30 micron sections of PFA perfusion fixed DRG tissue (on slide). Staining visualised using anti goat FITC secondary.

Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded rat cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

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IHC-Fr image of nNOS staining on marmoset caudate sections using ab1376 (1:1000). The tissue was fixed in paraformaldehyde and the sections were then permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab1376 was diluted 1:1000 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Rabbit polyclonal to Goat IgG (undiluted)

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Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded mouse cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

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Lane 1 : Anti-nNOS (neuronal) antibody (ab1376) at 2 µg/ml
Lane 2 : Anti-nNOS (neuronal) antibody (ab1376) at 1 µg/ml

Lane 1 : Human frontal cortex lysate (35 µg protein in RIPA buffer)
Lane 2 : Human brain sub ventricular zone lysate (35 µg protein in RIPA buffer)

Predicted band size: 161 kDa

Detected by chemiluminescence.

Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded dog cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrieval was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

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Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Incuabted with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor^® 488 secondary antibody (4 µg/ml), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).

Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor^® 488 secondary antibody (4 µg/ml).

Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab1376 for 1 hour (10 µg/ml) followed by Alexa Fluor^® 488 secondary antibody (2 µg/ml).

IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor^® 488 secondary antibody.