- 数据表
- 具体的参考文献 (24)
- 实验方案
概述
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产品名称Anti-nNOS (neuronal)抗体
参阅全部 nNOS (neuronal) 一抗 -
描述山羊多克隆抗体to nNOS (neuronal)
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宿主Goat
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经测试应用适用于: IHC-FrFl, WB, IHC-FoFr, IHC-P, IHC-Frmore details
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种属反应性与反应: Mouse, Rat, Rabbit, Human, Common marmoset
预测可用于: Cow, Dog
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免疫原
Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.
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阳性对照
- WB: mouse brain lysate IHC: mouse DRG following spinal nerve ligation (2 weeks)
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab1376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FrFl | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45, 160 kDa (predicted molecular weight: 161 kDa).Can be blocked with Human nNOS (neuronal) peptide (ab22863). Lyse in RIPA buffer. |
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| IHC-FoFr | 1/300. Ovenight incubation at room temp. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 16263087 | |
| IHC-Fr | 1/1000. PubMed: 22589245 |
靶标
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功能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
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组织特异性Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
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序列相似性Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
结构域The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
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翻译后修饰Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
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细胞定位Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
- Information by UniProt
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数据库链接
- Entrez Gene: 477498 Dog
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Entrez Gene: 24598 Rat
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
see all -
别名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded marmoset cerebellum tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of Sophie Pezet, King's College London, United Kingdomab1376 nNos antibody immunostaining nNos in dorsal root ganglion (DRG) neurons of an animal who had a spinal nerve ligation 2 weeks previous. [A] Image taken X10 objective, [B]Image taken with X20 objective; the staining is restricted to a subpopulation of neurons and is localized in the cytoplasm of these cells; Scale bar=20 microns. ab1376 was used at 1/300 overnight incubation at room temperature on 30 micron sections of PFA perfusion fixed DRG tissue (on slide). Staining visualised using anti goat FITC secondary. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded rat cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
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Immunohistochemistry (Frozen sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an anonymous abreview.IHC-Fr image of nNOS staining on marmoset caudate sections using ab1376 (1:1000). The tissue was fixed in paraformaldehyde and the sections were then permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab1376 was diluted 1:1000 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Rabbit polyclonal to Goat IgG (undiluted)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded mouse cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
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Western blot - Anti-nNOS (neuronal) antibody (ab1376)ab1376 (0.3 µg/ml) staining nNOS (neuronal) in human muscle whole tissue lysate (35 µg in RIPA). Incubation for 1 hour; detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nNOS (neuronal) antibody (ab1376)This image is courtesy of an Abreview submitted by Carl Hobbs.Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded dog cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrieval was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.
文献
This product has been referenced in:
- Guilbaud M et al. miR-708-5p and miR-34c-5p are involved in nNOS regulation in dystrophic context. Skelet Muscle 8:15 (2018). Read more (PubMed: 29703249) »
- Abot A et al. Galanin enhances systemic glucose metabolism through enteric Nitric Oxide Synthase-expressed neurons. Mol Metab 10:100-108 (2018). Read more (PubMed: 29428595) »
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