重组Anti-NLRP3抗体[EPR23073-96] (ab270449)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23073-96] to NLRP3
- Suitable for: IP, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-NLRP3抗体[EPR23073-96]
参阅全部 NLRP3 一抗 -
描述
兔单克隆抗体[EPR23073-96] to NLRP3 -
宿主
Rabbit -
特异性
NLRP3 is related with inflammation and is cell-type dependent. This antibody detects no signal or very weak signal in most normal natural samples. Stimulation may be required to allow detection of the NLRP3 protein in most samples, as described in the literature (PMID: 29070054, 29434227, and 22569257).
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经测试应用
适用于: IP, WB, ICC/IFmore details
不适用于: Flow Cyt,IHC-Fr or IHC-P -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: RAW 264.7 (+/- treatment with LPS) and J774A.1 whole cell lysates. ICC/IF: RAW 264.7 (+ treatment with LPS) cells. IP: RAW 264.7 (+ treatment with LPS) whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23073-96 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab270449于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
1/30.
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WB | (1) |
1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
Stimulation may be required to allow detection of the NLRP3 protein in most samples. There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs. |
ICC/IF |
1/50.
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说明 |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). Stimulation may be required to allow detection of the NLRP3 protein in most samples. There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs. |
ICC/IF
1/50. |
靶标
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功能
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. -
组织特异性
Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. -
疾病相关
Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. -
序列相似性
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 216799 Mouse
- SwissProt: Q8R4B8 Mouse
- Unigene: 54174 Mouse
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别名
- AGTAVPRL antibody
- AII/AVP antibody
- Angiotensin/vasopressin receptor AII/AVP like antibody
see all
图片
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All lanes : Anti-NLRP3 antibody [EPR23073-96] (ab270449) at 1/1000 dilution
Lane 1 : Raw 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Mouse thymus tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse kidney tissue lysate
Lane 6 : Mouse brain tissue lysate
Lane 7 : Mouse placenta tissue lysate
Lane 8 : Mouse ovary tissue lysate
Lane 9 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDaNLRP3 is expressed at a much low level in normal tissues (PMID: 33538177, PMID: 25524927, PMID: 20688930, PMID: 35676979, PMID: 31923221, PMID: 24166187).
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Lane1-8:180 seconds
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All lanes : Anti-NLRP3 antibody [EPR23073-96] (ab270449) at 1/1000 dilution
Lane 1 : Wild-type LPS treated (3 hour, 50 ng/mL) Mouse BMDCs
Lane 2 : NLRP3 knockout LPS treated (3 hour, 50 ng/mL) Mouse BMDCs
Lysates/proteins at 25000 cells per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Exposure time: 2 minutes
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (untreated)(-) and RAW 264.7 (+LPS 10ug/ml 8h)(+) cells labelling NLRP3 with ab270449 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 after treatment with LPS. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
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NLRP3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) (treated with 10 ug/ml Lipopolysaccharides (LPS) for 8 hours) whole cell lysate with ab270449 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270449 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 treated with 10 ug/ml Lipopolysaccharides (LPS) for 8 hours, whole cell lysate 10 ug
Lane 2: ab270449 IP in RAW 264.7 treated with 10 ug/ml Lipopolysaccharides(LPS) for 8 hours, whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270449 in RAW 264.7 treated with 10 ug/ml Lipopolysaccharides(LPS) for 8 hours, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds.
The band around 75kDa corresponds to the short form of NLRP3.
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All lanes : Anti-NLRP3 antibody [EPR23073-96] (ab270449) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : RAW 264.7 treated with 10 µg/ml Lipopolysaccharides (LPS) for 8 hours whole cell lysate
Lane 3 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight/expression profile observed is consistent with what has been described in the literature (PMID:26939933, 30315268 ).
The band around 75kDa corresponds to the short form of NLRP3.
Exposure time: 54 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (13)
ab270449 被引用在 13 文献中.
- Zheng J et al. 3,4-Methylenedioxy-β-Nitrostyrene Alleviates Dextran Sulfate Sodium-Induced Mouse Colitis by Inhibiting the NLRP3 Inflammasome. Front Pharmacol 13:866228 (2022). PubMed: 35784693
- Yang B et al. Glucocorticoid induces GSDMD-dependent pyrolysis in PC12 cells via endoplasmic reticulum stress. PLoS One 17:e0274057 (2022). PubMed: 36048803
- Jiang DQ et al. SIRT3 expression alleviates microglia activation‑induced dopaminergic neuron injury through the mitochondrial pathway. Exp Ther Med 24:662 (2022). PubMed: 36168411
- Pinheiro Campos AC et al. Effect of Subthalamic Stimulation and Electrode Implantation in the Striatal Microenvironment in a Parkinson's Disease Rat Model. Int J Mol Sci 23:N/A (2022). PubMed: 36292973
- Yang BW et al. Flavonoid-Conjugated Gadolinium Complexes as Anti-Inflammatory Theranostic Agents. Antioxidants (Basel) 11:N/A (2022). PubMed: 36552678