重组Anti-NLP1抗体[EPR16545] (ab192609)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16545] to NLP1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-NLP1抗体[EPR16545]
参阅全部 NLP1 一抗 -
描述
兔单克隆抗体[EPR16545] to NLP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- 293, K562, HeLa, PC12, NIH 3T3, C6, RAW 264.7 cell lysates, Human fetal liver, Mouse brain, heart, kidney and spleen, Rat heart and brain lysates; Human kidney clear cell carcinoma, tonsil and Mouse lung tissues; HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16545 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- NIH/3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- K-562 whole cell lysate (ab7911)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab192609于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100.
|
|
IP |
1/40.
|
说明 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
IP
1/40. |
靶标
-
相关性
CG1 is required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope. -
细胞定位
Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Excluded from the nucleolus. -
数据库链接
- Entrez Gene: 11097 Human
- Entrez Gene: 231042 Mouse
- Entrez Gene: 499974 Rat
- SwissProt: O15504 Human
- SwissProt: Q8CIC2 Mouse
-
别名
- CG1 antibody
- NLP1 antibody
- Nucleoporin hCG1 antibody
see all
图片
-
Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NLP1 with ab192609 at 1/100 dilution (6.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue). Negative control also shown.
-
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
All lanes : Anti-NLP1 antibody [EPR16545] (ab192609) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse heart tissue lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Mouse spleen tissue lysate
Lane 9 : Rat brain tissue lysate
Lane 10 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa -
Anti-NLP1 antibody [EPR16545] (ab192609) at 1/1000 dilution + Human fetal liver tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa -
All lanes : Anti-NLP1 antibody [EPR16545] (ab192609) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa -
Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab192609 at 1/40 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab192609 尚未被引用在任何文献中。