Anti-NKG2A抗体(ab96319)
Key features and details
- Rabbit polyclonal to NKG2A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-NKG2A抗体
参阅全部 NKG2A 一抗 -
描述
兔多克隆抗体to NKG2A -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human NKG2A (N terminal). The exact sequence is proprietary. Carrier protein conjugated.
-
阳性对照
- WB: A431 and HepG2 cell lysate. ICC/IF: HeLa cells. IHC-P: H1299 xenograft.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96319于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/1000.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
|
说明 |
---|
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
靶标
-
功能
Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. -
组织特异性
Natural killer cells. -
序列相似性
Contains 1 C-type lectin domain. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3821 Human
- Omim: 161555 Human
- SwissProt: P26715 Human
- Unigene: 512576 Human
-
别名
- C lectin type II protein antibody
- CD159 antigen like family member A antibody
- CD159 antigen-like family member A antibody
see all
图片
-
Paraffin-embedded H1299 xenograft stained for NKG2A using ab96319 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15 mins.
-
All lanes : Anti-NKG2A antibody (ab96319) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeled for NKG2A using ab96319 at a 1/200 dilution in immunocytochemistry.
Bottom panel co-stained with Hoechst 33342.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab96319 尚未被引用在任何文献中。