Key features and details
- Goat polyclonal to NIPP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
参阅全部 NIPP1 一抗
经测试应用适用于: IHC-P, WBmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Cow, Dog
- WB: HeLa, HepG2, Jurkat and NIH3T3 nuclear lysates. IHC-P: Human thymus tissue.
This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody说明This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.
Our Abpromise guarantee covers the use of ab5300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product.
功能Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
组织特异性Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
序列相似性Contains 1 FHA domain.
结构域Has a basic N- and C-terminal and an acidic central domain.
翻译后修饰May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
细胞定位Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
- Information by UniProt
- Activator of RNA decay antibody
- ARD 1 antibody
- ARD-1 antibody
All lanes : Anti-NIPP1 antibody (ab5300) at 1 µg/ml
Lane 1 : HeLa nuclear lysate in RIPA buffer
Lane 2 : HepG2 nuclear lysate in RIPA buffer
Lane 3 : Jurkat nuclear lysate in RIPA buffer
Lane 4 : NIH3T3 nuclear lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDa
Primary incubation for 1 hour. Detected using chemiluminescence.
ab5300 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab5300 被引用在 1 文献中.
- Nuytten M et al. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene 27:1449-60 (2008). WB . PubMed: 17724462