描述JAK/STAT signaling抑制剂. Nitrofuran antibiotic.
生物学描述JAK/STAT signaling inhibitor. Nitrofuran antibiotic. Inhibits JAK2 and Tyk2 phosphorylation and subsequent downstream STAT3 inhibition (EC50 = 3 μM). Antidiarrheal. Shows little effect on Akt, NF-κB, JAK1, MAPK and Src signaling.
化学名称4-Hydroxybenzoic acid 2-[(5-nitro-2-furanyl)methylene]hydrazide
存放说明Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解度概述Soluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab120951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
FOX-NY cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of nifuroxazide (ab120951). Decreased expression of Stat3 (phospho Y705) (ab76315) in FOX-NY cells correlates with an increase in nifuroxazide concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab76315 at 1/20000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
This product has been referenced in:
- Fernandes MB et al. Caco-2 cells cytotoxicity of nifuroxazide derivatives with potential activity against Methicillin-resistant Staphylococcus aureus (MRSA). Toxicol In Vitro 26:535-40 (2012). Read more (PubMed: 22285235) »
- Nelson EA et al. Nifuroxazide inhibits survival of multiple myeloma cells by directly inhibiting STAT3. Blood 112:5095-102 (2008). Read more (PubMed: 18824601) »