Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2抗体(ab189174)
Key features and details
- Goat polyclonal to Nicotinic Acetylcholine Receptor beta 2/CHRNB2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2抗体
参阅全部 Nicotinic Acetylcholine Receptor beta 2/CHRNB2 一抗 -
描述
山羊多克隆抗体to Nicotinic Acetylcholine Receptor beta 2/CHRNB2 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Hamster, Cow, Dog, Pig, Monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor beta 2/CHRNB2 aa 350-450 (internal sequence). NP_000739.1
Database link: P17787 -
阳性对照
- Human cerebellum lysate; Human brain, cortex tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab189174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 57 kDa).
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. |
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 57 kDa). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
疾病相关
Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 517519 Cow
- Entrez Gene: 490443 Dog
- Entrez Gene: 101135472 Gorilla
- Entrez Gene: 1141 Human
- Entrez Gene: 11444 Mouse
- Entrez Gene: 100516491 Pig
- Entrez Gene: 54239 Rat
- Omim: 118507 Human
see all -
别名
- Acetylcholine receptor beta 2 neural antibody
- ACHB2_HUMAN antibody
- ACHN antibody
see all
图片
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Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab189174) at 1 µg/ml + Human cerebellum lysate (in RIPA buffer) at 35 µg
Predicted band size: 57 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling Nicotinic Acetylcholine Receptor beta 2/CHRNB2 with ab189174 at 3.75 µg/ml.
数据表及文件
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Datasheet download
文献 (2)
ab189174 被引用在 2 文献中.
- Wang Q et al. Nicotine Activating α4β2 Nicotinic Acetylcholine Receptors to Suppress Neuroinflammation via JAK2-STAT3 Signaling Pathway in Ischemic Rats and Inflammatory Cells. Mol Neurobiol 59:3280-3293 (2022). PubMed: 35297011
- Durham E et al. Direct Effects of Nicotine Exposure on Murine Calvaria and Calvarial Cells. Sci Rep 9:3805 (2019). PubMed: 30846819