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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Proteases

Anti-Nicastrin抗体(ab3444)

  • Datasheet
Submit a review Q&A (1)References (3)

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Western blot - Anti-Nicastrin antibody (ab3444)

    Key features and details

    • Rabbit polyclonal to Nicastrin
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Nicastrin抗体
      参阅全部 Nicastrin 一抗
    • 描述

      兔多克隆抗体to Nicastrin
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Rat, Human
    • 免疫原

      Synthetic peptide corresponding to Human Nicastrin aa 688-708.
      Sequence:

      CINAKADVLFIAPREPGAVSY


      (Peptide available as ab4977)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • Primary antibody说明

      Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Also found in the neurofibrillary lesions associated with Alzheimer’s disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer’s diseased patients.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Proteases
      • Stem Cells
      • Signaling Pathways
      • Notch
      • Cytoplasmic

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Nicastrin peptide (ab4977)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    Our Abpromise guarantee covers the use of ab3444 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa).Can be blocked with Nicastrin peptide (ab4977).

    靶标

    • 功能

      Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
    • 组织特异性

      Widely expressed.
    • 序列相似性

      Belongs to the nicastrin family.
    • 细胞定位

      Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession Q92542 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 23385 Human
      • Entrez Gene: 59287 Mouse
      • Entrez Gene: 289231 Rat
      • Omim: 605254 Human
      • SwissProt: Q92542 Human
      • SwissProt: P57716 Mouse
      • SwissProt: Q8CGU6 Rat
      • Unigene: 517249 Human
      • Unigene: 218203 Mouse
      • Unigene: 51975 Rat
      see all
    • 别名

      • Anterior pharynx defective 2 antibody
      • APH 2 antibody
      • APH2 antibody
      • ATAG1874 antibody
      • KIAA0253 antibody
      • Ncstn antibody
      • NCT antibody
      • NICA_HUMAN antibody
      • Nicastrin antibody
      • RP11 517F10.1 antibody
      • RP11517F101 antibody
      see all

    图片

    • Western blot - Anti-Nicastrin antibody (ab3444)
      Western blot - Anti-Nicastrin antibody (ab3444)

      Western blot detection of Nicastrin on mouse primary cultured neuronal cells using ab3444. 

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (3)

    发表研究结果有使用 ab3444?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3444 被引用在 3 文献中.

    • Pintchovski SA  et al. Evidence that enzyme processivity mediates differential Aß production by PS1 and PS2. Curr Alzheimer Res 10:4-10 (2013). WB . PubMed: 22950863
    • Steuble M  et al. Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport. Biol Open 1:761-74 (2012). PubMed: 23213470
    • Massey LK  et al. Ubiquilin regulates presenilin endoproteolysis and modulates gamma-secretase components, Pen-2 and nicastrin. Biochem J 391:513-25 (2005). PubMed: 15975090

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    I work with with Dictyostelium discoideum which has a diverged Nicastrin orthologue. I am interested in seeing ahead of time if the sequence in which any of your Nicastrin antibodies is against is somewhat conserved in my organism before I buy one to find out whether it is worth trying or not. If I send you the sequence, can you check this for me to see if the region where the antibody is against is conserved? I have pasted the sequence below: MYTSLNSYPCTRIMTLNGQIGCSSSHGGDSGILYLIDSDESYHNYFSYNQQKDIIVVFDS NYFNKTLVLEMYSKKKMNGALVLTDIGKTYPYSPEDQYPIKQFGLYPDSNLNWNPNGDGF TYMNFPFPMFALELKTSIIIRNLSTINRDGKYPAYGAELDSFMQGAINAETCLRRGFCEP VGGQSIWSSFSEVIDQSKPIILVMLPIDATAFFRDLATGTDQSGYALTVLLSMLNTLQGV DKTKWDKEVIFAMWNSERWGYVGSTNFVNDLLNFNCTSLDSNNQNSCSSPPMLDLTFEQI KFENIYAIIEFNQIGRPVNSGKKTPNKLDIYNLVFHPNGGAGANQLMDVFSQSTQSYENS TIQFQKTTQNELPPCSSMSFIKEINKKSAPNFIGTLVITDHDYQYNNPYFGDEQDNSGNI NTTTSTLFDMVQVFSKSIDLLAGGNGTVKVDDLFIREINVCLTQSITCNWVTKLMSTFPY NPIPNFYSGVYGVSPVNHITPIETRFIFRMATYLTQHRTNATNCTSDNDCDTSSSICVNK VCLYSNTHYHNAISLAFSFDNSKSSWTIVNTSYPVFVESNWDYTTVRLFQVGSYANEIWF LVSGLIELLLSVGIIFYIKKYLSKRYKLL*

    Read More

    Abcam community

    Verified customer

    Asked on Jan 30 2007

    Answer

    Thank you for your patience. I have received a reply from the originator of ab45425 (Nicastrin antibody). The immunogenic peptide sequence is proprietary information but they have performed a sequence alignment for you. Based on the alignment results, we know it is unlikely that this product will react with Dictyostelium discoideum and we do not recommend using ab45425 with this species. Please don't hesitate to contact us again if you have further questions.

    Read More

    Abcam Scientific Support

    回复于 Feb 01 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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