Anti-NFYA抗体(ab6558)
Key features and details
- Rabbit polyclonal to NFYA
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NFYA抗体
参阅全部 NFYA 一抗 -
描述
兔多克隆抗体to NFYA -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
This product was prepared from monospecific antiserum by delipidation and defibrination. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab6558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 39 kDa. |
靶标
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功能
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. -
序列相似性
Belongs to the NFYA/HAP2 subunit family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4800 Human
- Entrez Gene: 18044 Mouse
- Omim: 189903 Human
- SwissProt: P23511 Human
- SwissProt: P23708 Mouse
- Unigene: 10441 Human
- Unigene: 4929 Mouse
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别名
- CAAT box DNA binding protein subunit A antibody
- CAAT box DNA-binding protein subunit A antibody
- CBF A antibody
see all
图片
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Western blot - Anti-NFYA antibody (ab6558)This image is courtesy of an anonymous Abreview.Anti-NFYA antibody (ab6558) at 1/500 dilution + CaPan1 whole cell lysate at 50 µg
Secondary
HRP-conjugated Donkey anti-Rabbit. at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 40 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
文献 (7)
ab6558 被引用在 7 文献中.
- Amirteimouri S et al. Epigenetic role of the nuclear factor NF-Y on ID gene family in endometrial tissues of women with endometriosis: a case control study. Reprod Biol Endocrinol 17:32 (2019). PubMed: 30876429
- Hiramoto M et al. A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression. Int J Mol Med 41:717-728 (2018). WB ; Rat . PubMed: 29207083
- Ma H et al. ZHX2 enhances the cytotoxicity of chemotherapeutic drugs in liver tumor cells by repressing MDR1 via interfering with NF-YA. Oncotarget 6:1049-63 (2015). IP, ChIP ; Human . PubMed: 25473899
- Lagadec C et al. The RNA-binding protein Musashi-1 regulates proteasome subunit expression in breast cancer- and glioma-initiating cells. Stem Cells 32:135-44 (2014). PubMed: 24022895
- Zhang Z et al. Immune mediators regulate CFTR expression through a bifunctional airway-selective enhancer. Mol Cell Biol 33:2843-53 (2013). PubMed: 23689137
- Hou Y et al. DNA Demethylation and USF Regulate the Meiosis-Specific Expression of the Mouse Miwi. PLoS Genet 8:e1002716 (2012). EMSA ; Mouse . PubMed: 22661915
- Grskovic M et al. Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells. PLoS Genet 3:e145 (2007). EMSA ; Human . PubMed: 17784790
