Anti-NFAT1 (phospho S54)抗体(ab200819)
Key features and details
- Rabbit polyclonal to NFAT1 (phospho S54)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-NFAT1 (phospho S54)抗体
参阅全部 NFAT1 一抗 -
描述
兔多克隆抗体to NFAT1 (phospho S54) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse NFAT1 (phospho S54).
Database link: Q60591 -
阳性对照
- Murine T-cells + PMA + Ionomycin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab200819 was negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated NFAT1. The final product is generated by affinity chromatography using a NFAT1-derived peptide phosphorylated at serine 54. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab200819于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 100 kDa. |
靶标
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功能
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. -
组织特异性
Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. -
序列相似性
Contains 1 RHD (Rel-like) domain. -
结构域
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors. -
翻译后修饰
In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
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数据库链接
- Entrez Gene: 18019 Mouse
- SwissProt: Q60591 Mouse
- Unigene: 116802 Mouse
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别名
- AI607462 antibody
- cytoplasmic 2 antibody
- KIAA0611 antibody
see all
图片
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All lanes : Anti-NFAT1 (phospho S54) antibody (ab200819) at 1/1000 dilution
Lane 1 : Extracts of Murine T cells were left untreated with no peptide
Lane 2 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with no peptide
Lane 3 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with a generic phosphoserine-containing peptide
Lane 5 : Extracts of Murine T cells were treated with PMA and Ca2+ ionophore
ionomycin with the phosphopeptide immunogen
Secondary
All lanes : Goat F(ab’)2 anti-rabbit IgG alkaline phosphatase
Predicted band size: 100 kDaResolved by SDS-PAGE on a 10% Tris-glycine gel
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab200819 被引用在 5 文献中.
- Banerjee A et al. A reengineered common chain cytokine augments CD8+ T cell-dependent immunotherapy. JCI Insight 7:N/A (2022). PubMed: 35603788
- Quinn WJ et al. Lactate Limits T Cell Proliferation via the NAD(H) Redox State. Cell Rep 33:108500 (2020). PubMed: 33326785
- Wu L et al. FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine 41:384-394 (2019). PubMed: 30803933
- Molinari F et al. The mitochondrial metabolic reprogramming agent trimetazidine as an 'exercise mimetic' in cachectic C26-bearing mice. J Cachexia Sarcopenia Muscle 8:954-973 (2017). PubMed: 29130633
- Zhang X et al. Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells. Mol Cancer 16:147 (2017). PubMed: 28851360