Application
Western blot
Sample
Human Cell lysate - whole cell (Human THP-1 macrophages)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
70 µg
Treatment
100 µg/ml oxidized LDL (oxLDL) +/- 10 µg/ml apoA-I, 24h
Specification
Human THP-1 macrophages
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST plus 5% skim milk
Dilution
1/50000
Detection
Detection method
ECL+
Negative control
None.
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 65 kDa
Positive control
None.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Anca Sima
Verified customer
提交于 Jun 04 2015