Anti-Neuropeptide Y抗体(ab6173)

Reviews (1)Q&A (3)References (13)

Key features and details

  • Sheep polyclonal to Neuropeptide Y
  • Reacts with: Rabbit, Guinea pig, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Neuropeptide Y抗体
    参阅全部 Neuropeptide Y 一抗

  • 描述

    羊多克隆抗体to Neuropeptide Y

  • 宿主

    Sheep

  • 特异性

    The specificity of the NPY antiserum was determined by radioimmunoassay. Synthetic peptides, somatostatin-14, leu-enkephalin, met-enkephalin, oxytocin, Arg8-vasopressin, vasoactive intestinal peptide, angiotensin II demonstrated less than 0.005% cross- reactivity and substance P less than 0.02%. Peptide YY and bovine pancreatic polypeptide demonstrated approx. 36% and 0.05% cross- reactivity respectively. Human peptide YY share 65% homology amino acid sequence for human NPY. Synthetic NPY completely abolished radio labelled NPY binding to NPY antibody in RIA and antibody binding to tissue antigen.

  • 种属反应性

    与反应: Rabbit, Guinea pig, Human

  • 免疫原

    Synthetic peptide:

    TPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRY

    , corresponding to amino acids 30-64 of Human Neuropeptide Y. The peptide was coupled to Bovine Thyroglobulin.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

靶标

  • 功能

    NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

  • 组织特异性

    One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

  • 序列相似性

    Belongs to the NPY family.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • C-flanking peptide of NPY antibody
      • CPON antibody
      • Neuropeptide tyrosine antibody
      • Neuropeptide Y precursor antibody
      • NPY antibody
      • NPY_HUMAN antibody
      • Pro neuropeptide Y antibody
      • PYY 4 antibody
      • PYY4 antibody
      • Y Neuropeptide antibody
      see all

    文献 (13)

    发表研究结果有使用 ab6173？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6173 被引用在 13 文献中.

    • Shu Q  et al. Acupuncture Targeting SIRT1 in the Hypothalamic Arcuate Nucleus Can Improve Obesity in High-Fat-Diet-Induced Rats with Insulin Resistance via an Anorectic Effect. Obes Facts 13:40-57 (2020). PubMed: 31935731
    • Damasceno S  et al. Top Common Differentially Expressed Genes in the Epileptogenic Nucleus of Two Strains of Rodents Susceptible to Audiogenic Seizures: WAR and GASH/Sal. Front Neurol 11:33 (2020). PubMed: 32117006
    • Robinson SL  et al. Medial prefrontal cortex neuropeptide Y modulates binge-like ethanol consumption in C57BL/6J mice. Neuropsychopharmacology 44:1132-1140 (2019). PubMed: 30647448
    • Huang X  et al. Effects of electroacupuncture on luteal regression and steroidogenesis in ovarian hyperstimulation syndrome model rat. Life Sci 197:1-9 (2018). WB . PubMed: 29482802
    • Wang R  et al. Neuropeptide Y-Positive Neurons in the Dorsomedial Hypothalamus Are Involved in the Anorexic Effect of Angptl8. Front Mol Neurosci 11:451 (2018). PubMed: 30618603
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Neuropeptide Y antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: r.t°C
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.2% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Dr. Fabio Canneva

    Verified customer

    提交于 Jul 10 2014

    Question

    can I use the antibody on formaline fixed paraffine embbeded tissues?

    Read More
    Answer

    We cannot guarantee this antibody works on paraffin embedded tissues. It has not been tested for this.

    Read More

    Question

    Dear Abcam: This antibody said to provide good staining in rabbit and guinea pig. Is it also good for staining of rat brain? Are there any other references to rat brain? Thank you

    Read More
    Answer

    This antibody has not been tested for staining rat brain, so its reactivity with rat NPY is unknown, although certainly possible. You may be able to estimate the likelihood of cross-reactivity with this species by comparing the sequence of the synthetic peptide immunogen with the sequences of rat, rabbit and guinea pig NPY.

    Read More

    Question

    I have 2 questions 1. What is the affinity constant for the antibody. 2. What is the concentration of antibody in a sample. Thank you.

    Read More
    Answer

    The affinity constant for this antibody has not been determined. The concentration of antibody in the sample has not been determined and the material is supplied as a whole serum. This specific antibody has been used for the past 15 or so years for many publications including those listed in the datasheet and the dilution quoted on the datasheet is accurate (1/5000 working dilution).

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com