Neurokinin A,Tachykinin多肽(ab120185)
Key features and details
- Tachykinin peptide
- CAS Number: 86933-74-6
- Soluble in water
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Neurokinin A,Tachykinin多肽 -
描述
Tachykinin多肽 -
别名
- NKA
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生物学描述
Endogenous tachykinin peptide
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CAS编号
86933-74-6 -
化学结构
性能
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分子量
1133.32 -
分子式
C50H80N14O14S -
序列
HKTDSFVGLM (Modifications: C-terminal amide) -
PubChem识别号
55582 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120185于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120185, Neurokinin A, Tachykinin peptide
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K562 cells were incubated at 37°C for 4h with vehicle control (0 μM) and 15 nM of neurokinin A (ab120185). Increased expression of p53 (ab26) correlates with an increase in neurokinin A concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab26 at 5 μg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120185 被引用在 1 文献中.
- Drimousis S et al. Gender-Related Differences of Tachykinin NK2 Receptor Expression and Activity in Human Colonic Smooth Muscle. J Pharmacol Exp Ther 375:28-39 (2020). PubMed: 32764152