Anti-Neurogenin 2/NGN2抗体(ab154293)
Key features and details
- Rabbit polyclonal to Neurogenin 2/NGN2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-Neurogenin 2/NGN2抗体
参阅全部 Neurogenin 2/NGN2 一抗 -
描述
兔多克隆抗体to Neurogenin 2/NGN2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Mouse Neurogenin 2/NGN2 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: P70447 -
阳性对照
- This antibody gave a positive signal in the following tissue lysates: Mouse E10 Embryonic Brain; Rat E14 Embryonic Brain; Rat E14 Embryonic Spinal Cord; Rat E16 Embryonic Brain; Rat E16 Embryonic Spinal Cord. This antibody gave a positive result when used in the following methanol fixed cell lines: PC12
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Matched antibody pairs
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154293于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
靶标
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功能
Involved in neurogenesis. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 11924 Mouse
- Entrez Gene: 295475 Rat
- SwissProt: P70447 Mouse
- Unigene: 42017 Mouse
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别名
- Atoh 4 antibody
- Atoh4 antibody
- bHLHa8 antibody
see all
图片
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ab154293 staining Neurogenin 2/NGN2 in Rat Primary Neurons DIV1 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab154293 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Neurogenin 2/NGN2 antibody (ab154293) at 1 µg/ml (Milk blocking 5%)
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : E14 Rat Embryo Brain Tissue Lysate
Lane 3 : E14 Rat Embryo Spinal Cord Tissue Lysate
Lane 4 : E16 Rat Embryo Brain Tissue Lysate
Lane 5 : E16 Rat Embryo Spinal Cord Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab154293 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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All lanes : Anti-Neurogenin 2/NGN2 antibody (ab154293) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : E14 Rat Embryo Brain Tissue Lysate
Lane 3 : E14 Rat Embryo Spinal Cord Tissue Lysate
Lane 4 : E16 Rat Embryo Brain Tissue Lysate
Lane 5 : E16 Rat Embryo Spinal Cord Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Additional bands at: 100 kDa (possible non-specific binding), 150 kDa (possible non-specific binding), 48 kDa (possible non-specific binding)
Exposure time: 90 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154293 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab154293 被引用在 1 文献中.
- Wei SW et al. Role of the hydrogen sulfide-releasing donor ADT-OH in the regulation of mammal neural precursor cells. J Cell Physiol 237:2877-2887 (2022). PubMed: 35342944