Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioma cell line C6)
Specification
glioma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5%
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse TRITC conjugated T7782
Host species: Goat
Clonality: Polyclonal
Conjugation: TRITC
Host species: Goat
Clonality: Polyclonal
Conjugation: TRITC
Dilution
1/300
Additional data
Additional Notes
Nucleii are counterstaind with DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 31 2011