概述

  • 产品名称
    Anti-Neurocan抗体[650.24]
    参阅全部 Neurocan 一抗
  • 描述
    小鼠单克隆抗体[650.24] to Neurocan
  • 宿主
    Mouse
  • 特异性
    Specific for Neurocan core protein; also reacts with neurocan C fragment
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Embryonic rat brain proteoglycans

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    650.24
  • 骨髓瘤
    P3x63
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 143 kDa.

靶标

图片

  • ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.

    See Abreview

文献

This product has been referenced in:
  • Li G  et al. Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats. Front Cell Neurosci 10:241 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27807407) »
See 1 Publication for this product

客户评价及客户问答

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

According to the Unigen expression profile of this protein, a good positive control for this protein could be brain or vascular mouse tissue lysates.

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.169047

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioblastoma cell line C6)
Specification
glioblastoma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioma cell line C6)
Specification
glioma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5%

Abcam user community

Verified customer

提交于 Aug 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line D54MG)
Specification
human glioblastoma cell line D54MG
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X 100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Aug 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册