重组Alexa Fluor® 568荧光Anti-NeuN抗体[EPR12763] - Neuronal Marker (ab207282)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 568 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
- Suitable for: ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 568荧光Anti-NeuN抗体[EPR12763] - Neuronal Marker
参阅全部 NeuN 一抗 -
描述
Alexa Fluor® 568荧光兔单克隆抗体[EPR12763] to NeuN - Neuronal Marker -
宿主
Rabbit -
偶联物
Alexa Fluor® 568. Ex: 578nm, Em: 603nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Sheep, Goat, Cat, Dog, Human, Zebrafish, Common marmoset -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: PC12 cell and B35 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12763 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
- Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)
- Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
- Biotin Anti-NeuN antibody [EPR12763] (ab204681)
- Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207279)
- Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207281)
- Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)
- FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab207282于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
1/100.
This product gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
说明 |
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ICC/IF
1/100. This product gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
靶标
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功能
RNA-binding protein that regulates alternative splicing events. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 101084840 Cat
- Entrez Gene: 608803 Dog
- Entrez Gene: 102186905 Goat
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- Entrez Gene: 101111982 Sheep
- Entrez Gene: 100538330 Zebrafish
see all -
别名
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
图片
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ab207282 staining NeuN in B35 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207282 at 1/100 dilution (pseudocolored in green) and ab190573, Rabbit monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This product also gave a positive signal under the same testing conditions in B35 cells fixed with 100% methanol (5 min).
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ab207282 staining NeuN in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207282 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in PC12 cells fixed with 4% formaldehyde (10 min).
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab207282 被引用在 5 文献中.
- Ratz M et al. Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics. Nat Neurosci 25:285-294 (2022). PubMed: 35210624
- Miao Y et al. 9-Cyanopyronin probe palette for super-multiplexed vibrational imaging. Nat Commun 12:4518 (2021). PubMed: 34312393
- Zhao Z et al. Parallel, minimally-invasive implantation of ultra-flexible neural electrode arrays. J Neural Eng 16:035001 (2019). PubMed: 30736013
- Jones ME et al. Hippocampal interleukin-1 mediates stress-enhanced fear learning: A potential role for astrocyte-derived interleukin-1ß. Brain Behav Immun 67:355-363 (2018). PubMed: 28963000
- Jones ME et al. Chemogenetic Manipulation of Dorsal Hippocampal Astrocytes Protects Against the Development of Stress-enhanced Fear Learning. Neuroscience N/A:N/A (2018). PubMed: 30030056