Application
Western blot
Sample
Human Cell lysate - whole cell (hiPSC)
Gel Running Conditions
Reduced Non-Denaturing (Native) (15%)
Loading amount
25 µg
Specification
hiPSC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL solution
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45-50 kDa Non-specific: 30 kDa
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提交于 Oct 21 2020