Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Coronal Brain Sections)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X in PBS
Specification
Coronal Brain Sections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: incubated overnight
Dilution
1/500
Additional data
Additional Notes
Image is 40x magnification of the dentate gyrus granule neurons located in a 20um coronal section of rat hippocampus.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Kyle Klingbeil
Verified customer
提交于 Aug 12 2015