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Neuroscience Cell Type Marker Neuron marker Soma marker
重组RabMAb

重组Anti-NeuN抗体[EPR12763] - Neuronal Marker (ab177487)

  • Datasheet
  • SDS
Reviews (66) Submit a question References (294)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Multiplex immunohistochemistry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (PFA fixed) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Flow Cytometry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
  • Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
  • Suitable for: IHC (PFA fixed), mIHC, Flow Cyt, IHC-P, WB, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat, Sheep, Goat, Dog, Human, Zebrafish, Common marmoset

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概述

  • 产品名称

    Anti-NeuN抗体[EPR12763] - Neuronal Marker
    参阅全部 NeuN 一抗
  • 描述

    兔单克隆抗体[EPR12763] to NeuN - Neuronal Marker
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC (PFA fixed)
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Rat
    Sheep
    Goat
    Dog
    Human
    Zebrafish
    mIHC
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: A6NFN3

  • 阳性对照

    • WB: Mouse brain, mouse cerebellum, rat cerebellum and human fetal brain tissue lysates. ICC/IF: SH-SY-5Y and Mouse primary neuron cells. IHC-P: Human cerebellum, human gliocytoma tissue. mIHC: Human cerebellum tissue IHC-Fr: Mouse dentate gyrus tissue. Flow Cyt: U-87 MG cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR12763
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Signal Transduction
    • Antibodies
    • neun

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)
    • Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
    • Biotin Anti-NeuN antibody [EPR12763] (ab204681)
    • Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207279)
    • Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207281)
    • Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207282)
    • Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)
    • FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • ioGlutamatergic Neurons - Human iPSC-Derived Glutamatergic Neurons (ab259259)
    • Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    • Anti-Alpha-synuclein antibody [LB 509] (ab27766)
    • Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IHC (PFA fixed)
Mouse
IHC-Fr
Mouse
IHC-P
Mouse
Rat
Sheep
Goat
Dog
Human
Zebrafish
mIHC
Human
WB
Mouse
Rat
Human
All applications
Cat
Pig
Cynomolgus monkey
应用 Ab评论 说明
IHC (PFA fixed)
Use at an assay dependent concentration.
mIHC
1/1000.

Perform Sodium citrate antigen retrieval (pH 6.0) in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (16)
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/800.

WB (6)
1/1000 - 1/10000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/2000.

ICC/IF (11)
1/100 - 1/300.

For unpurified use at 1/80.

IHC-Fr (16)
Use at an assay dependent concentration.
说明
IHC (PFA fixed)
Use at an assay dependent concentration.
mIHC
1/1000.

Perform Sodium citrate antigen retrieval (pH 6.0) in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/800.

WB
1/1000 - 1/10000. Detects a band of approximately 48,50 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/2000.

ICC/IF
1/100 - 1/300.

For unpurified use at 1/80.

IHC-Fr
Use at an assay dependent concentration.

靶标

  • 功能

    RNA-binding protein that regulates alternative splicing events.
  • 序列相似性

    Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession A6NFN3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 101084840 Cat
    • Entrez Gene: 608803 Dog
    • Entrez Gene: 102186905 Goat
    • Entrez Gene: 146713 Human
    • Entrez Gene: 52897 Mouse
    • Entrez Gene: 287847 Rat
    • Entrez Gene: 101111982 Sheep
    • Entrez Gene: 100538330 Zebrafish
    • SwissProt: A6NFN3 Human
    • SwissProt: Q8BIF2 Mouse
    • Unigene: 135229 Human
    • Unigene: 341103 Mouse
    • Unigene: 143966 Rat
    see all
  • 别名

    • FLJ56884 antibody
    • FLJ58356 antibody
    • Fox-1 homolog C antibody
    • fox1 homolog C antibody
    • Fox3 antibody
    • FOX3NeuN antibody
    • hexaribonucleotide binding protein 3 antibody
    • HRNBP3 antibody
    • NEUN antibody
    • neuronal nuclei antibody
    • Rbfox3 antibody
    • RFOX3_HUMAN antibody
    • RNA binding protein fox-1 homolog 3 antibody
    • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling NeuN with ab177487 at 1/100 dilution (B), SOX1 with ab242125 at 1/100 dilution (C) and Olig2 with ab109186 at 1/100 dilution (D). Anti-Rabbit and Mouse Polymer HRP was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Heat mediated antigen retrieval (Leica ER2, PH9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibodies from the previous round, to avoid any cross-reactivity. 

    Panel A: merged staining of anti- NeuN (green, Opal™520), anti-SOX1 (red, Opal™570) and anti- Olig2 (yellow, Opal™690).

    Panel B: anti-NeuN stands for neurons.

    Panel C: anti-SOX1 stained on neural progenitors.

    Panel D: anti-Olig2 stained on oligodendrocyte.

    The section was incubated in three rounds of staining: in the order of ab177487, ab242125 and ab109186 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

     

  • Multiplex immunohistochemistry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Multiplex immunohistochemistry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Fluorescence multiplex immunohistochemical analysis of human cerebellum tissue (formalin-fixed paraffin-embedded section).

    Merged staining of Neu-N (ab177487; yellow; Opal™570), anti-beta III Tubulin (ab52623; red; Opal™690) and anti-GFAP (ab68428; green; Opal™520).

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ kit.

    The section was incubated in three rounds of staining with ab177487 (1/1000 dilution), ab52623 (1/200 dilution) and ab68428 (1/250 dilution); each using a separate fluorescent tyramide signal amplification system.

    Sodium citrate antigen retrieval (pH 6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

    DAPI (blue) was used as a nuclear counter stain.

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunofluorescence staining of NeuN using ab177487 in ioNEURONS/glut cells (Human iPSC-Derived Glutamatergic Neurons, ab259259), which were differentiated for 1 day post induction.

    The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab177487 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunocytochemistry/immunofluorescence analysis of Mouse primary neuron cells labelling NeuN with ab177487 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 mouse monoclonal antibody (ab11267) at 1/200 dilution and visualised using Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

    Confocal image showing mainly nuclear staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of NeuN (green) and GFAP (red) double staining on mouse cerebellum sections using ab177487 (1/5000) and ab4674 (1/1500) respectively.

    The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were then incubated with Rabbit Monoclonal to NeuN (ab177487) diluted at 1/5000 and Chicken Polyclonal to GFAP (ab4674) diluted at 1/1500. The primary antibody was detected using ab150097 Goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (1/500) and ab150176 Goat anti-chicken IgY conjugated to Alexa Fluor® 594 (1/500)

  • Immunohistochemistry (PFA fixed) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (PFA fixed) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    NeuN antibody ab177487 was used with Tissue Clearing Kit ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI, Green: NeuN.

    Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.

    For 1 mm brain sections, we recommend a starting dilution of 1:200, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (ab150077) at a dilution of 1:400.

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an Abreview submitted by Vladimir Milenkovic

    Immunocytochemistry/immunofluorescence analysis of human neurons differentiated from iPSCs labelling NeuN (green) with ab177487 at 1/500 in 0.1% TritonX-100, 1% goat serum, 1X PBS for 16 hours at 4°C. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Then, cells were blocked with 5% serum for 20 minutes at 23°C. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody. Tuj1 antibody was used to stain neuronal dendrites and axons (red).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    ​An independent comparison of commercially available NeuN clones in IHC-P.

    Competitor A: Leading mouse monoclonal.

    Competitor B: Non-Abcam rabbit monoclonal.

    Sodium citrate was used for antigen retrieval in all 3 samples.

    ab177487 produces specific staining, equivalent to the leading mouse monoclonal at half the dilution. The non-Abcam mouse monoclonal was less specific as it stained Purkinje cells, which do not express NeuN.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    IHC image of NeuN (ab177487) with Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) staining in formalin fixed paraffin embedded normal human cerebellum tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (ab177487, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, Anti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866, 1.0µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)Zhao et al PLoS One. 2016 Apr 5;11(4):e0152551. doi: 10.1371/journal.pone.0152551. eCollection 2016. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemsitry analysis of neurons labeling nuclei with NeuN.

    Primary cortical neurons were prepared from the cortices of 1-day-old newborn pups. Briefly, the cortices were dissected in cold PBS. Tissues were collected and washed in PBS, and 0.05% (v/v) trypsin was added for digestion at 37°C for 15 minutes. The digestion was stopped by the addition of fetal bovine serum to a final concentration of 10% (v/v). Cells were collected by centrifugation at 800 x g for 10 minutes to remove the PBS and were resuspended in Neurobasal medium supplemented with 2% (v/v) B27.

    Neurons plated out were rinsed with PBS three times and then fixed in 4% paraformaldehyde for 25 minutes at 4°C. Fixed cells were incubated in 0.1% (v/v) sodium citrate contain 0.1% (v/v) Triton X-100 for 2 minutes on ice, and then neurons were washed twice with PBS and incubated with 300 μl ab177487 (1:500 in PBS containing 10% goat serum) for 2 hours at 37°C. An Alexa-Fluor® 594-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (4',6-diamidino-2-phenylindole) was added for 10 minutes at room temperature followed by PBS washing to fluorescently label nuclei. Samples were photographed using a fluorescence microscope (LEICA DMI3000, Japan) and analysed using the Leica application suite.

    Neuronal nuclei were labeled with ab177487 (top left panel, red), while all cells nuclei were stained in blue (top right panel).

    (Purity of cultured mouse cortical neuron was 93.00% ± 1.23% at 7 days in vitro).

  • Flow Cytometry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Flow Cytometry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Overlay histogram showing U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells stained with ab177487 (red line).

    The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab177487, 1/100 dilution) for 30 minutes at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 1μg/1x106 cells used under the same conditions. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Alexa Fluor® 488 (ab190195) and Alexa Fluor® 647 (ab190565) conjugated versions are available for this clone.

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunocytochemsitry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling NeuN (green) with ab177487 at 1/300. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    All lanes : Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/10000 dilution (purified)

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    Exposure time -
    Lane 1-2: 3 minutes.
    Lane 3-4: 1 minute.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    ​An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections).

    Competitor A: Leading mouse monoclonal.

    Competitor B: Non-Abcam rabbit monoclonal.

    ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.

  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an Abreview submitted by Jianning Lu

    ab177487 staining NeuN in mouse free floating 50 micron lumbar spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 10% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS + Triton) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/700) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Dr. Ryan MacDonald (Cambridge University, United Kingdom)

    IHC-Fr staining of NeuN on zebrafish brain tissue at 4 days post-fertilization using ab177487 (1/100). The sections were fixed in paraformaldehyde and permeabilized using triton X. Antigen retrieval uisng sodium citrate was used. The sections were blocked using 5% BSA for 1 hour at 23°C. ab177487 was diluted 1/100 and incubated for 16 hours at 4°C. The secondary antibody used was anti rabbit IgG conjugated to Alexa Fluor® 488 (1/1000). DAPI used as counterstain.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an Abreview submitted by Eva Borger

    ab177487 staining NeuN in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with Triton X-100 + 0.4% horse seurm for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on cat cerebellum sections using ab177487 (1/1000).

    Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on dog cerebellum sections using ab177487 (1/500).

    Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling NeuN with ab177487 at 1/3000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on rat brain (SVZ) sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on mouse brain (frontal cortex) sections using ab177487 (1/800). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/800 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on zebrafish spinal cord sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on marmoset cerebellum sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on sheep brain (Frontal cortex) sections using ab177487 (1/1000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

    IHC-P image of FOX3/NeuN staining on goat cerebellum sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    See Abreview

  • Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (294)

    发表研究结果有使用 ab177487?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab177487 被引用在 294 文献中.

    • Liu MH  et al. Differential neuronal reprogramming induced by NeuroD1 from astrocytes in grey matter versus white matter. Neural Regen Res 15:342-351 (2020). PubMed: 31552908
    • Zhou H  et al. Salvianolic acid B activates Wnt/ß-catenin signaling following spinal cord injury. Exp Ther Med 19:825-832 (2020). PubMed: 32010242
    • Liu X  et al. Subdural haematomas drain into the extracranial lymphatic system through the meningeal lymphatic vessels. Acta Neuropathol Commun 8:16 (2020). PubMed: 32059751
    • Wang S  et al. Danhong Injection Protects Hemorrhagic Brain by Increasing Peroxiredoxin 1 in Aged Rats. Front Pharmacol 11:346 (2020). PubMed: 32292340
    • Long B  et al. Mapping the Architecture of Ferret Brains at Single-Cell Resolution. Front Neurosci 14:322 (2020). PubMed: 32351352
    View all Publications for this product

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    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
    Sample
    Mouse Tissue sections (Embryonic day 15 brain)
    Specification
    Embryonic day 15 brain
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 16 2014

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Coronal Brain Sections)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X in PBS
    Specification
    Coronal Brain Sections
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Kyle Klingbeil

    Verified customer

    提交于 Aug 12 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: r.t°C
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.2% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Dr. Fabio Canneva

    Verified customer

    提交于 Jul 09 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 4°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
    Sample
    Mouse Tissue sections (Mouse brain)
    Specification
    Mouse brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Adam Walker

    Verified customer

    提交于 Jun 19 2014

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.5% Triton X
    Fixative
    Paraformaldehyde
    Read More

    Sumit Sarkar

    Verified customer

    提交于 May 21 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-NeuN [EPR12763] antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Horse Seurm/ 0.4% Tx100 as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Sample
    Mouse Tissue sections (Brain tissue)
    Specification
    Brain tissue
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Ms. Eva Borger

    Verified customer

    提交于 May 15 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOX3/NeuN [EPR12763] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ctric acid
    Sample
    Cat Tissue sections (Cerebellum)
    Specification
    Cerebellum
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Oct 29 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human iPSC neurons)
    Permeabilization
    Yes - 1% TritonX-100
    Specification
    Human iPSC neurons
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 23 2021

    Western blot abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hiPSC)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (15%)
    Loading amount
    25 µg
    Specification
    hiPSC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Abcam user community

    Verified customer

    提交于 Oct 21 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-NeuN antibody [EPR12763] - Neuronal Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (brain sections)
    Permeabilization
    No
    Specification
    brain sections
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

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    提交于 Oct 21 2020

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