Anti-NET1抗体(ab5914)

Submit a review Q&A (1)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NET1 antibody (ab5914)

    Key features and details

    • Goat polyclonal to NET1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-NET1抗体
      参阅全部 NET1 一抗

    • 描述

      山羊多克隆抗体to NET1

    • 宿主

      Goat

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide: DKALSGGKRKETLV, corresponding to C terminal amino acids 529-542 of Human NET1.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      GenBank Accession Number – NP_005854

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab5914 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    靶标

    • 功能

      Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

    • 组织特异性

      Widely expressed.

    • 序列相似性

      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.

    • 细胞定位

      Cytoplasm. Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • ARHG8_HUMAN antibody
        • ARHGEF8 antibody
        • GLYT2 antibody
        • Guanine nucleotide regulatory protein oncogene antibody
        • MGC114559 antibody
        • MGC127767 antibody
        • MGC53602 antibody
        • mNET1 antibody
        • Net1 antibody
        • NET1A antibody
        • Neuroepithelial cell transforming gene 1 antibody
        • Neuroepithelial cell-transforming gene 1 protein antibody
        • p65 Net1 proto oncogene antibody
        • Proto-oncogene p65 Net1 antibody
        • Rho guanine nucleotide exchange factor 8 antibody
        • Rho guanine nucleotide exchange factor GEF 8 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NET1 antibody (ab5914)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NET1 antibody (ab5914)

        ab5914 (2.5 µg/ml) staining NET1 in Human Tonsil by IHC-P.  Steamed antigen retrieval with citrate buffer pH 6, AP-staining.  This image shows staining of fibrous structures.

      文献 (5)

      发表研究结果有使用 ab5914？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab5914 被引用在 5 文献中.

      • Sun X  et al. Inhibition of NET-1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway. Exp Ther Med 17:2334-2340 (2019). PubMed: 30867719
      • Levi L  et al. Bacterial genotoxins promote inside-out integrin ß1 activation, formation of focal adhesion complexes and cell spreading. PLoS One 10:e0124119 (2015). WB . PubMed: 25874996
      • Srougi MC & Burridge K The Nuclear Guanine Nucleotide Exchange Factors Ect2 and Net1 Regulate RhoB-Mediated Cell Death after DNA Damage. PLoS One 6:e17108 (2011). WB ; Human . PubMed: 21373644
      • Dutertre M  et al. Estrogen regulation and physiopathologic significance of alternative promoters in breast cancer. Cancer Res 70:3760-70 (2010). WB ; Human . PubMed: 20406972
      • García-Mata R  et al. The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. Mol Cell Biol 27:8683-97 (2007). PubMed: 17938206

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      Question

      Hello, I am studying zebrafish Net1 and am therefore interested in a Net1 antibody. However, in terms of the peptide sequence your antibody is directed against, the only identitical residues shared with the zebrafish protein are GKRKET. Since you have not tested cross reactivity of your antibody with zebrafish, I was wondering whether I could get a small sample of it to test for cross reactivity in the fish on a Western blot. If it does cross react then I would be very interested in purchasing it. Thank you very much, Kathy

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      Answer

      We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

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