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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-NET1抗体(ab5914)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

Product price, shipping and contact information

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Key features and details

  • Goat polyclonal to NET1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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概述

  • 产品名称

    Anti-NET1抗体
    参阅全部 NET1 一抗
  • 描述

    山羊多克隆抗体to NET1
  • 宿主

    Goat
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide: DKALSGGKRKETLV, corresponding to C terminal amino acids 529-542 of Human NET1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    GenBank Accession Number – NP_005854

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5914于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
  • 组织特异性

    Widely expressed.
  • 序列相似性

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • 细胞定位

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q7Z628 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10276 Human
    • SwissProt: Q7Z628 Human
    • Unigene: 25155 Human
    • 别名

      • ARHG8_HUMAN antibody
      • ARHGEF8 antibody
      • GLYT2 antibody
      • Guanine nucleotide regulatory protein oncogene antibody
      • MGC114559 antibody
      • MGC127767 antibody
      • MGC53602 antibody
      • mNET1 antibody
      • Net1 antibody
      • NET1A antibody
      • Neuroepithelial cell transforming gene 1 antibody
      • Neuroepithelial cell-transforming gene 1 protein antibody
      • p65 Net1 proto oncogene antibody
      • Proto-oncogene p65 Net1 antibody
      • Rho guanine nucleotide exchange factor 8 antibody
      • Rho guanine nucleotide exchange factor GEF 8 antibody
      see all

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab5914?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5914 被引用在 5 文献中.

    • Sun X  et al. Inhibition of NET-1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway. Exp Ther Med 17:2334-2340 (2019). PubMed: 30867719
    • Levi L  et al. Bacterial genotoxins promote inside-out integrin ß1 activation, formation of focal adhesion complexes and cell spreading. PLoS One 10:e0124119 (2015). WB . PubMed: 25874996
    • Srougi MC & Burridge K The Nuclear Guanine Nucleotide Exchange Factors Ect2 and Net1 Regulate RhoB-Mediated Cell Death after DNA Damage. PLoS One 6:e17108 (2011). WB ; Human . PubMed: 21373644
    • Dutertre M  et al. Estrogen regulation and physiopathologic significance of alternative promoters in breast cancer. Cancer Res 70:3760-70 (2010). WB ; Human . PubMed: 20406972
    • García-Mata R  et al. The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. Mol Cell Biol 27:8683-97 (2007). PubMed: 17938206

    客户评价及客户问答

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    Question

    Hello, I am studying zebrafish Net1 and am therefore interested in a Net1 antibody. However, in terms of the peptide sequence your antibody is directed against, the only identitical residues shared with the zebrafish protein are GKRKET. Since you have not tested cross reactivity of your antibody with zebrafish, I was wondering whether I could get a small sample of it to test for cross reactivity in the fish on a Western blot. If it does cross react then I would be very interested in purchasing it. Thank you very much, Kathy

    Read More

    Abcam community

    Verified customer

    Asked on Dec 30 2003

    Answer

    We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Abcam Scientific Support

    回复于 Dec 30 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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