Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse cortical culture)
Permeabilization
Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
Specification
mouse cortical culture
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 27°C · Diluent: 2% donkey serum in 0.2% TritonX 100
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey Anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Lenin Veeraval
Verified customer
提交于 May 31 2021