Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Adult hippocampal brain sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - 1% Triton
Specification
Adult hippocampal brain sections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 2% Donkey Serum + 0.2% Triton
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488 donkey anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
The nestin antibody beautifully stains neural stem cells in the dentate gyrus of the adult hippocampus--a primary site of neurogenesis in the adult brain. The antibody labels both the cell body and the neurite process.
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提交于 Sep 26 2008