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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments

Anti-Nestin抗体[Rat-401] - Neural Stem Cell Marker (ab6142)

  • Datasheet
  • SDS
Reviews (28)Q&A (8)References (180)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
  • Western blot - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)

Key features and details

  • Mouse monoclonal [Rat-401] to Nestin - Neural Stem Cell Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

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概述

  • 产品名称

    Anti-Nestin抗体[Rat-401] - Neural Stem Cell Marker
    参阅全部 Nestin 一抗
  • 描述

    小鼠单克隆抗体[Rat-401] to Nestin - Neural Stem Cell Marker
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Sheep, Cat, Monkey
  • 免疫原

    Tissue, cells or virus corresponding to Rat Nestin. Homogenized spinal cord tissue from embryonic day 15 (E15) rats.

  • 阳性对照

    • WB: Mouse brain and rat brain whole tissue lysates. IHC-P: Rat Brain 6 weeks (cerebellum sagittal, coronal rest) tissue sections.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    Rat-401
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Signal Transduction
    • Antibodies
    • nestin
    • Neuroscience
    • Development

相关产品

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)
    • HRP Anti-Nestin antibody [rat-401] (ab196694)
    • Alexa Fluor® 488 Anti-Nestin antibody [rat-401] (ab197495)
    • Anti-Nestin antibody [Rat 401] - BSA and Azide free (ab264111)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6142于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (8)
Use a concentration of 0.05 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.

WB (3)
Use a concentration of 1 µg/ml. Predicted molecular weight: 200 kDa.

Block with milk or BSA but do not dilute primary antibody in buffer containing milk.

说明
IHC-P
Use a concentration of 0.05 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.

WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 200 kDa.

Block with milk or BSA but do not dilute primary antibody in buffer containing milk.

靶标

  • 功能

    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • 组织特异性

    CNS stem cells.
  • 序列相似性

    Belongs to the intermediate filament family.
  • 发展阶段

    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • 翻译后修饰

    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 18008 Mouse
    • SwissProt: Q6P5H2 Mouse
    • SwissProt: P21263 Rat
    • Unigene: 331129 Mouse
    • Unigene: 9701 Rat
    • 别名

      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)

      IHC image of Nestin staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6142, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
      Western blot - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
      All lanes : Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142) at 1 µg/ml

      Lane 1 : Mouse E12 brain tissue lysate
      Lane 2 : Mouse E14 brain tissue lysate
      Lane 3 : Mouse E16 brain tissue lysate
      Lane 4 : Mouse E18 brain tissue lysate
      Lane 5 : Rat E12 brain tissue lysate
      Lane 6 : Rat E14 brain tissue lysate
      Lane 7 : Rat E16 brain tissue lysate
      Lane 8 : Rat E18 brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 200 kDa



      This blot was produced using a 3-8% Tris-Acetate gel under the Tris-Acetate buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab6142 overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)Image from Lu L. et al., Sci Rep. 2018 Aug 14;8(1):12083. Fig2b. doi: 10.1038/s41598-018-29441-3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

      REST expression in N-hREST mouse brains correlates with stemness in embryonic neural stem cells. Immunofluorescence analysis of E18.5 N-hREST and LSL-hRESTcontrol littermate mouse brains with antibodies against REST (using an antibody that preferentially recognizes hREST over mouse REST) and Nestin (using ab6142)

      Mice were anesthetized and perfused with phosphate-buffered saline followed by 4% paraformaldehyde (PFA). Brain tissues were then dissected and fixed in 4% PFA overnight at 4 °C. Fixed brain tissues were processed for paraffin embedding and then cut into 5-μm sections. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)This image is courtesy of an anonymous abreview.

      ab6142 staining adult mouse brain tissue section by Immunohistochemistry (Formalin/PFA-fixed, paraffin embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Sodium Citrate, permeabilization in 1% Triton buffer and blocking in 10% serum for 1 hour at 25°C. The primary antibody, diluted 1/200 (PBS, 2% Donkey serum, 0.2% Triton) for 16 hours at 4°C.  An Alexa Fluor® 488 conjugated donkey polyclonal to mouse Ig, diluted 1/500 was used as the secondary.

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (180)

    发表研究结果有使用 ab6142?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6142 被引用在 180 文献中.

    • Peng T  et al. miR-125/CDK2 axis in cochlear progenitor cell proliferation. Mol Med Rep 23:N/A (2021). PubMed: 33300064
    • Jia J  et al. Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1. Stem Cell Res Ther 12:169 (2021). PubMed: 33691791
    • Xiao Z  et al. Pimozide augments bromocriptine lethality in prolactinoma cells and in a xenograft model via the STAT5/cyclin D1 and STAT5/Bcl-xL signaling pathways. Int J Mol Med 47:113-124 (2021). PubMed: 33155660
    • Recabal A  et al. The FGF2-induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic-dependent pathway. J Neurochem 156:182-199 (2021). PubMed: 32936929
    • Song D  et al. GABAB receptor antagonist promotes hippocampal neurogenesis and facilitates cognitive function recovery following acute cerebral ischemia in mice. Stem Cell Res Ther 12:22 (2021). PubMed: 33413637
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 36 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse cortical culture)
    Permeabilization
    Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
    Specification
    mouse cortical culture
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Lenin Veeraval

    Verified customer

    提交于 May 31 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 23 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 23 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nestin antibody [Rat 401] - Neural Stem Cell Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0, 20 mins 95C
    Permeabilization
    Yes - 0.05% TritonX
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 28 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Nestin antibody [Rat 401] - Neural Stem Cell Marker

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - Triton-X 100
    Specification
    Brain
    Blocking step
    Donkey serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Alyssa Cortella

    Verified customer

    提交于 Aug 24 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Nestin antibody [Rat 401] - Neural Stem Cell Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - TritonX-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Kyungjoo Seong

    Verified customer

    提交于 Nov 02 2016

    Immunocytochemistry abreview for Anti-Nestin antibody [Rat 401] - Neural Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Mouse neural stem cell)
    Permeabilization
    Yes - tritonx100
    Specification
    Mouse neural stem cell
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Kyungjoo Seong

    Verified customer

    提交于 Nov 02 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Nestin antibody [Rat 401] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Permeabilization
    Yes - Triton
    Specification
    brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 09 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Nestin antibody [Rat 401] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (adult rat heart tissue)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    adult rat heart tissue
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 28 2016

    Western blot abreview for Anti-Nestin antibody [Rat 401] - Neural Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    400000 cells
    Treatment
    Serum deprivation (0.1%) for 24 hrs
    Specification
    Mouse Neuroblastoma (Neuro2A)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 25 2015

    1-10 of 36 Abreviews or Q&A

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