Key features and details
- Mouse monoclonal [Rat-401] to Nestin - Neural Stem Cell Marker
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG1
产品名称Anti-Nestin抗体[Rat-401] - Neural Stem Cell Marker
参阅全部 Nestin 一抗
描述小鼠单克隆抗体[Rat-401] to Nestin - Neural Stem Cell Marker
经测试应用适用于: IHC-P, WBmore details
预测可用于: Mouse不与反应: Sheep, Cat, Human, Monkey
Tissue, cells or virus corresponding to Rat Nestin. Homogenized spinal cord tissue from embryonic day 15 (E15) rats.
- WB: Mouse brain and rat brain whole tissue lysates. IHC-P: Rat Brain 6 weeks (cerebellum sagittal, coronal rest) tissue sections.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab6142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.05 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 200 kDa.
Block with milk or BSA but do not dilute primary antibody in buffer containing milk.
功能Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
组织特异性CNS stem cells.
序列相似性Belongs to the intermediate filament family.
发展阶段Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
翻译后修饰Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
- Information by UniProt
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IHC image of Nestin staining in a section of formalin-fixed paraffin-embedded normal rat brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab6142, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142) at 1 µg/ml
Lane 1 : Mouse E12 brain tissue lysate
Lane 2 : Mouse E14 brain tissue lysate
Lane 3 : Mouse E16 brain tissue lysate
Lane 4 : Mouse E18 brain tissue lysate
Lane 5 : Rat E12 brain tissue lysate
Lane 6 : Rat E14 brain tissue lysate
Lane 7 : Rat E16 brain tissue lysate
Lane 8 : Rat E18 brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 200 kDa
This blot was produced using a 3-8% Tris-Acetate gel under the Tris-Acetate buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab6142 overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.
REST expression in N-hREST mouse brains correlates with stemness in embryonic neural stem cells. Immunofluorescence analysis of E18.5 N-hREST and LSL-hRESTcontrol littermate mouse brains with antibodies against REST (using an antibody that preferentially recognizes hREST over mouse REST) and Nestin (using ab6142)
Mice were anesthetized and perfused with phosphate-buffered saline followed by 4% paraformaldehyde (PFA). Brain tissues were then dissected and fixed in 4% PFA overnight at 4 °C. Fixed brain tissues were processed for paraffin embedding and then cut into 5-μm sections.
ab6142 staining adult mouse brain tissue section by Immunohistochemistry (Formalin/PFA-fixed, paraffin embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Sodium Citrate, permeabilization in 1% Triton buffer and blocking in 10% serum for 1 hour at 25°C. The primary antibody, diluted 1/200 (PBS, 2% Donkey serum, 0.2% Triton) for 16 hours at 4°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse Ig, diluted 1/500 was used as the secondary.
ab6142 被引用在 132 文献中.
- Kim JE et al. Single cell and genetic analyses reveal conserved populations and signaling mechanisms of gastrointestinal stromal niches. Nat Commun 11:334 (2020). PubMed: 31953387
- Zhao M et al. Direct reprogramming adult fibroblast into cells with partial inner ear hair cell characteristics through cell activation and signal directed approach. Neurosci Lett 729:135010 (2020). PubMed: 32344104
- Ma QQ et al. Bone marrow mesenchymal stem cells modified by angiogenin-1 promotes tissue repair in mice with oxygen-induced retinopathy of prematurity by promoting retinal stem cell proliferation and differentiation. J Cell Physiol 234:21027-21038 (2019). PubMed: 31106420
- Wang YM et al. MicroRNA-197 controls ADAM10 expression to mediate MeCP2's role in the differentiation of neuronal progenitors. Cell Death Differ 26:1863-1879 (2019). PubMed: 30560934
- Kong YL et al. Silencing of the MEKK2/MEKK3 Pathway Protects against Spinal Cord Injury via the Hedgehog Pathway and the JNK Pathway. Mol Ther Nucleic Acids 17:578-589 (2019). PubMed: 31382189