Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 0.1% Triton
Specification
Brain
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Fixative
Methanol
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS with 2.5% BSA
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: alexa fluor 680 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Additional data
Additional Notes
A few nestin positive cells in green located in the hilus of the dentate gyrus of an adult rat. For higher magnification, this antibody looks best in frozen sections.
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提交于 Nov 18 2008