Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Human Tissue sections (human tumor bearing mouse brain)
Specification
human tumor bearing mouse brain
Blocking step
bsa 1% + Goat serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: goat serum 5%
Dilution
1/500
Secondary antibody
Name
Abcam antibody:Goat Anti-Mouse IgG1 heavy chain (FITC) preadsorbed
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Francoise Geffroy
Verified customer
提交于 May 02 2014