Key features and details
- Rabbit polyclonal to Nedd8
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
参阅全部 NEDD8 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Saccharomyces cerevisiae
Recombinant full length protein (S. cerevisiae).
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯化说明Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Our Abpromise guarantee covers the use of ab4751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 9.7 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions.
功能Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
组织特异性Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
序列相似性Belongs to the ubiquitin family.
翻译后修饰Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
细胞定位Nucleus. Mainly nuclear.
- Information by UniProt
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ab4751 tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of Donkey polyclonal to Rabbit IgG (HRP) (ab7083) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). ab4751 tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of Donkey polyclonal to Rabbit IgG (HRP) (ab7083) for 1 hour at r
Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody generated by immunization with recombinant yeast Rub1 was tested by immunoblot against yeast lysates expressing the Rub1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (ab7125). Panel C shows specific reaction with Rub1 using a 1:1,000 dilution of ab4751 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat mi