Anti-NEAS抗体(ab2558)
Key features and details
- Rabbit polyclonal to NEAS
- Suitable for: WB
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
概述
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产品名称
Anti-NEAS抗体
参阅全部 NEAS 一抗 -
描述
兔多克隆抗体to NEAS -
宿主
Rabbit -
特异性
This antibody recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human -
免疫原
Synthetic peptide corresponding to Human NEAS. Surrounding amino acid 162.
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常规说明
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab2558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 4 µg/ml. |
靶标
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功能
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
疾病相关
Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia. -
序列相似性
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats. -
翻译后修饰
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. - Information by UniProt
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数据库链接
- Entrez Gene: 374234 Chicken
- Entrez Gene: 6709 Human
- Entrez Gene: 64159 Rat
- Omim: 182810 Human
- SwissProt: P07751 Chicken
- SwissProt: Q13813 Human
- SwissProt: P16546 Mouse
- SwissProt: P16086 Rat
see all -
别名
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
see all
图片
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Western blot - Anti-NEAS antibody (ab2558)All lanes : Anti-NEAS antibody (ab2558) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 285 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 71 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-NEAS antibody (ab2558)Anti-NEAS antibody (ab2558) at 4 µg/ml + HeLa cell extract
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible cleavage fragment)
文献 (4)
ab2558 被引用在 4 文献中.
- Woods LT et al. P2X7 receptor activation induces inflammatory responses in salivary gland epithelium. Am J Physiol Cell Physiol 303:C790-801 (2012). WB ; Mouse . PubMed: 22875784
- Grubb DR et al. Phospholipase C{beta}1b associates with a Shank3 complex at the cardiac sarcolemma. FASEB J 25:1040-7 (2011). WB ; Rat . PubMed: 21148417
- Kraner SD et al. Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical illness myopathy. Am J Physiol Regul Integr Comp Physiol 300:R1384-91 (2011). WB ; Rat . PubMed: 21474431
- Végran F et al. A short caspase-3 isoform inhibits chemotherapy-induced apoptosis by blocking apoptosome assembly. PLoS One 6:e29058 (2011). WB . PubMed: 22216167
