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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Crosslinking

Anti-NEAS抗体(ab2558)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (4)

Product price, shipping and contact information

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Western blot - Anti-NEAS antibody (ab2558)
  • Western blot - Anti-NEAS antibody (ab2558)

Key features and details

  • Rabbit polyclonal to NEAS
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Chicken, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-NEAS抗体
    参阅全部 NEAS 一抗
  • 描述

    兔多克隆抗体to NEAS
  • 宿主

    Rabbit
  • 特异性

    This antibody recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to Human NEAS. Surrounding amino acid 162.

  • 常规说明

     This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab2558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 4 µg/ml.

靶标

  • 功能

    Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • 疾病相关

    Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • 序列相似性

    Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • 翻译后修饰

    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • 细胞定位

    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Target information above from: UniProt accession Q13813 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 374234 Chicken
    • Entrez Gene: 6709 Human
    • Entrez Gene: 64159 Rat
    • Omim: 182810 Human
    • SwissProt: P07751 Chicken
    • SwissProt: Q13813 Human
    • SwissProt: P16546 Mouse
    • SwissProt: P16086 Rat
    • Unigene: 372331 Human
    • Unigene: 204969 Mouse
    • Unigene: 5812 Rat
    see all
  • 别名

    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

图片

  • Western blot - Anti-NEAS antibody (ab2558)
    Western blot - Anti-NEAS antibody (ab2558)
    All lanes : Anti-NEAS antibody (ab2558) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 285 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 60 kDa, 71 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-NEAS antibody (ab2558)
    Western blot - Anti-NEAS antibody (ab2558)
    Anti-NEAS antibody (ab2558) at 4 µg/ml + HeLa cell extract

    Observed band size: 250 kDa why is the actual band size different from the predicted?
    Additional bands at: 150 kDa (possible cleavage fragment)

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (4)

    发表研究结果有使用 ab2558?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2558 被引用在 4 文献中.

    • Woods LT  et al. P2X7 receptor activation induces inflammatory responses in salivary gland epithelium. Am J Physiol Cell Physiol 303:C790-801 (2012). WB ; Mouse . PubMed: 22875784
    • Grubb DR  et al. Phospholipase C{beta}1b associates with a Shank3 complex at the cardiac sarcolemma. FASEB J 25:1040-7 (2011). WB ; Rat . PubMed: 21148417
    • Kraner SD  et al. Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical illness myopathy. Am J Physiol Regul Integr Comp Physiol 300:R1384-91 (2011). WB ; Rat . PubMed: 21474431
    • Végran F  et al. A short caspase-3 isoform inhibits chemotherapy-induced apoptosis by blocking apoptosome assembly. PLoS One 6:e29058 (2011). WB . PubMed: 22216167

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    Hello, The spec sheet of ab2558 says that the immunogen is “Synthetic peptide surrounding amino acid 162 of mouse alpha-Fodrin”. Contradicting to this description, in the Scientific Support section of this production, it is declared in answers to Question 2-5 that the immunogen is Synthetic peptide surrounding a.a 1190 of human alpha-fodrin. Also, in Question 1 it is claimed that it does not recognize mouse. The descriptions above are contradicting each other. Could you please carefully look into the product and let me know what's going on? We are working on this antibody right now, and do need to know what's the actual situation. Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2012

    Answer

    Thank you for your enquiry. I have investigated our records for ab2558 Alpha Fodrin antibody. Regarding mouse reactivity, the answer to this question on the datasheet was provided in 2006. We now have information to confirm that this antibody has been tested succesfully in mouse, and so we now have mouse as a tested and guaranteed species. Regarding the immunogen, again these enquires were from 2004 to 2006. I can confirm that the immunogen is a synthetic peptide surrounding amino acid 162 of mouse alpha-Fodrin. I have confirmed this with our source. This appears to have changed in the past and I am currently investigating this with them. I understand your confusion, and I have removed these published enquires from the datasheet. (Please note it can take a few days for this to update on the internet Cache systems). I apologize for any inconvenience. The quality of our antibodies and datasheets is very important to us, as is any feedback and data we receive. Should you have any further questions or concerns regarding this issue, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Jan 10 2012

    Western blot abreview for Anti-NEAS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293,HELA and JURKAT)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    10 µg
    Specification
    HEK293,HELA and JURKAT
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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