Anti-NEAS抗体(ab2558)
Key features and details
- Rabbit polyclonal to NEAS
- Suitable for: WB
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
概述
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产品名称
Anti-NEAS抗体
参阅全部 NEAS 一抗 -
描述
兔多克隆抗体to NEAS -
宿主
Rabbit -
特异性
This antibody recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human -
免疫原
Synthetic peptide corresponding to Human NEAS. Surrounding amino acid 162.
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常规说明
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab2558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 4 µg/ml. |
靶标
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功能
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
疾病相关
Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia. -
序列相似性
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats. -
翻译后修饰
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. - Information by UniProt
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数据库链接
- Entrez Gene: 374234 Chicken
- Entrez Gene: 6709 Human
- Entrez Gene: 64159 Rat
- Omim: 182810 Human
- SwissProt: P07751 Chicken
- SwissProt: Q13813 Human
- SwissProt: P16546 Mouse
- SwissProt: P16086 Rat
see all -
别名
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
see all
图片
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Western blot - Anti-NEAS antibody (ab2558)All lanes : Anti-NEAS antibody (ab2558) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 285 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 71 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-NEAS antibody (ab2558)Anti-NEAS antibody (ab2558) at 4 µg/ml + HeLa cell extract
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible cleavage fragment)
文献 (4)
ab2558 被引用在 4 文献中.
- Woods LT et al. P2X7 receptor activation induces inflammatory responses in salivary gland epithelium. Am J Physiol Cell Physiol 303:C790-801 (2012). WB ; Mouse . PubMed: 22875784
- Grubb DR et al. Phospholipase C{beta}1b associates with a Shank3 complex at the cardiac sarcolemma. FASEB J 25:1040-7 (2011). WB ; Rat . PubMed: 21148417
- Kraner SD et al. Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical illness myopathy. Am J Physiol Regul Integr Comp Physiol 300:R1384-91 (2011). WB ; Rat . PubMed: 21474431
- Végran F et al. A short caspase-3 isoform inhibits chemotherapy-induced apoptosis by blocking apoptosome assembly. PLoS One 6:e29058 (2011). WB . PubMed: 22216167
