重组Anti-NDUFS6抗体[EPR15957] (ab195807)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15957] to NDUFS6
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-NDUFS6抗体[EPR15957]
参阅全部 NDUFS6 一抗 -
描述
兔单克隆抗体[EPR15957] to NDUFS6 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal heart, Human skeletal muscle, Mouse brain, Mouse heart, Rat brain, Rat heart and Rat kidney lysates; Human tonsil, Human hepatocellular carcinoma and Rat liver tissues; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15957 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab195807于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/1000.
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说明 |
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WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/1000. |
靶标
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功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
序列相似性
Belongs to the complex I NDUFS6 subunit family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4726 Human
- Entrez Gene: 407785 Mouse
- Entrez Gene: 29478 Rat
- Omim: 603848 Human
- SwissProt: O75380 Human
- SwissProt: P52503 Mouse
- SwissProt: P52504 Rat
- Unigene: 408257 Human
see all -
别名
- BC059730 antibody
- CI 13kA antibody
- CI 13kD A antibody
see all
图片
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Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/10000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 13 kDa -
Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/10000 dilution + Human fetal heart at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 13 kDa -
Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 13 kDa -
All lanes : Anti-NDUFS6 antibody [EPR15957] (ab195807) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 13 kDa -
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Inset image: negative control obtained using PBS instead of ab195807 and secondary antibody only.
Note: Cytoplasm staining on human tonsil tissue was observed.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.
Inset image: negative control obtained using PBS instead of ab195807 and secondary antibody only.
Note: Cytoplasm staining on human hepatocellular carcinoma tissue was observed.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.
Inset image: negative control obtained using PBS instead of ab195807 and secondary antibody only.
Note: Cytoplasm staining on rat liver tissue was observed.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunoprecipitation analysis of Human fetal kidney lysate labeling NDUFS6 using ab195807 at 1/30 dilution (Lane 2).
Lane 3: IP using Rabbit monoclonal IgG (ab172730) ) instead of ab195807 in Human fetal kidney lysates.
Lane 1: Input: 10 μg Human fetal kidney lysates.
Subsequent WB detection was performed using ab195807 at 1/1000 dilution.
An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab195807 被引用在 4 文献中.
- D'Angelo L et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep 35:109002 (2021). PubMed: 33882309
- Gao M et al. Mitoflash generated at the Qo site of mitochondrial Complex III. J Cell Physiol 236:2920-2933 (2021). PubMed: 32930405
- Stokum JA et al. A large portion of the astrocyte proteome is dedicated to perivascular endfeet, including critical components of the electron transport chain. J Cereb Blood Flow Metab 41:2546-2560 (2021). PubMed: 33818185
- Rouzier C et al. NDUFS6 related Leigh syndrome: a case report and review of the literature. J Hum Genet N/A:N/A (2019). PubMed: 30948790