Name: Anti-Ndufs4 antibody [EP7832]
Brand: RabMAb
SKU: ab137064
Rating: 5 (6 reviews)

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Western blot - Anti-Ndufs4 antibody [EP7832] (ab137064)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP7832] to Ndufs4
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

产品名称

Anti-Ndufs4抗体[EP7832]
参阅全部 Ndufs4 一抗
描述

兔单克隆抗体[EP7832] to Ndufs4
宿主

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Rat
WB	Mouse Rat Human

See all applications and species data

免疫原

Synthetic peptide within Human Ndufs4 aa 150-250 (C terminal). The exact sequence is proprietary.
阳性对照
- 293T cell lysates, fetal brain and fetal stomach tissue lysates; Human brain and Human stomach tissues Mouse heat lysate, rat heart lysate, mouse kidney, HeLa.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP7832
同种型

IgG
研究领域

Alternative Versions
- Anti-Ndufs4 antibody [EP7832] - BSA and Azide free (ab232337)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Ndufs4 protein (ab86684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Rat
WB	Mouse Rat Human

应用	Ab评论	说明
WB	(1)	1/500 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
IHC-P	(3)	1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF	(2)	1/500. For unpurified use at 1/50 - 1/100.
IP		1/10 - 1/100.
Flow Cyt		1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurifed use at 1/100 - 1/1000 dilution.

说明
WB 1/500 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500. For unpurified use at 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurifed use at 1/100 - 1/1000 dilution.

功能

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
疾病相关

Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
序列相似性

Belongs to the complex I NDUFS4 subunit family.
细胞定位

Mitochondrion inner membrane.
Target information above from: UniProt accession O43181 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 4724 Human
- Entrez Gene: 17993 Mouse
- Entrez Gene: 499529 Rat
- Omim: 602694 Human
- SwissProt: O43181 Human
- SwissProt: Q9CXZ1 Mouse
- SwissProt: Q5XIF3 Rat
- Unigene: 528222 Human
- Unigene: 253142 Mouse
- Unigene: 203141 Rat
see all
别名
- AQDQ antibody
- CI 18 antibody
- CI 18 kDa antibody
- CI AQDQ antibody
- CI-18 kDa antibody
- CI-AQDQ antibody
- Complex I 18 kDa antibody
- Complex I AQDQ antibody
- Complex I-18 kDa antibody
- Complex I-AQDQ antibody
- mitochondrial antibody
- mitochondrial respiratory chain complex I (18 KD subunit) antibody
- NADH coenzyme Q reductase antibody
- NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody
- NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody
- NADH dehydrogenase antibody
- NADH ubiquinone oxidoreductase 18 kDa subunit antibody
- NADH-ubiquinone oxidoreductase 18 kDa subunit antibody
- NDUFS4 antibody
- NDUS4_HUMAN antibody
see all

Western blot - Anti-Ndufs4 antibody [EP7832] (ab137064)

Anti-Ndufs4 antibody [EP7832] (ab137064) at 1/50000 dilution (purified) + Rat heart lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 20 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-Ndufs4 antibody [EP7832] (ab137064)

ab137064 (purified) at 1:30 dilution (2μg) immunoprecipitating Ndufs4 in Rat heart lysate.

Lane 1 (input): Rat heart lysate, 10μg
Lane 2 (+): ab137064 & Rat heart lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137064 in Rat heart lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-Ndufs4 antibody [EP7832] (ab137064)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ndufs4 with purified ab137064 at 1:60 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-Ndufs4 antibody [EP7832] (ab137064)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ndusf5 with Purified ab137064 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab7291 anti-Tubulin (mouse mAb) ab150120 AlexaFluor ® 594 Goat anti-Mouse secondary (1:1000,2 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (ab137064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Ndufs4 with Purified ab137064 at 1:50 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (ab137064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Ndufs4 with Purified ab137064 at 1:50 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (ab137064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labeling Ndufs4 with Purified ab137064 at 1:50 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Ndufs4 antibody [EP7832] (ab137064)

All lanes : Anti-Ndufs4 antibody [EP7832] (ab137064) at 1/2000 dilution (purified)

Lane 1 : Human fetal brain lysates
Lane 2 : Mouse heart lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Ndufs4 antibody [EP7832] (ab137064)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Ndufs4 knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: Human fetal heart tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab137064 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

Unpurified ab137064 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab137064 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (ab137064)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Ndufs4 with unpurified ab137064 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Ndufs4 antibody [EP7832] (ab137064)

Overlay histogram showing HepG2 cells stained with unpurified ab137064 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137064, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-Ndufs4 antibody [EP7832] (ab137064)

All lanes : Anti-Ndufs4 antibody [EP7832] (ab137064) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysate
Lane 2 : Fetal brain tissue lysate
Lane 3 : Fetal kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EP7832] (ab137064)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Ndufs4 with unpurified ab137064 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Ndufs4 antibody [EP7832] (ab137064)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab137064？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab137064 被引用在 1 文献中.

Piekutowska-Abramczuk D et al. NDUFB8 Mutations Cause Mitochondrial Complex I Deficiency in Individuals with Leigh-like Encephalomyopathy. Am J Hum Genet 102:460-467 (2018). PubMed: 29429571

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK-293)

Gel Running Conditions

Non-reduced Non-Denaturing (Native) (8-12% Bis-Tris)

Loading amount

40 µg

Specification

HEK-293

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

提交于 Jan 25 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (human induced pluripotent stem cell derived cardio)

Permeabilization

Yes - 0.02% Triton X

Specification

human induced pluripotent stem cell derived cardio

Blocking step

Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 27°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 Jan 25 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HEK293)

Permeabilization

Yes - 0.5% Triton X-100

Specification

HEK293

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C

Fixative

Neutral buffered formalin

Abcam user community

Verified customer

提交于 May 20 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Kidney

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

Mr. Carl Hobbs

Verified customer

提交于 Feb 11 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Kidney

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

Mr. Carl Hobbs

Verified customer

提交于 Feb 11 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citric acid

Permeabilization

Specification

Kidney

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

Fixative

Formaldehyde

Mr. Carl Hobbs

Verified customer

提交于 Feb 11 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

概述

产品名称

描述

宿主

Tested Applications & Species

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (1)

客户评价及客户问答

Western blot abreview for Anti-Ndufs4 antibody [EP7832]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ndufs4 antibody [EP7832]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ndufs4 antibody [EP7832]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ndufs4 antibody [EP7832]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ndufs4 antibody [EP7832]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ndufs4 antibody [EP7832]