Mouse, Rat, Chicken, Cow, Monkey
Recombinant fragment, corresponding to a region within amino acids 60-264 of Human NDUFS3 (Uniprot ID: O75489).
THP1 and HL-60 whole cell lysates. U87 xenograft.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
1/100 - 1/1000.
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Belongs to the complex I 30 kDa subunit family.
Mitochondrion inner membrane.
Information by UniProt
CI 30 antibody
CI 30KD antibody
Western blot - Anti-NDUFS3 antibody (ab154543)
All lanes : Anti-NDUFS3 antibody (ab154543) at 1/1000 dilution Lane 1 : THP1 whole cell lysate Lane 2 : HL60 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 30 kDa 12% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody (ab154543)
Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling NDUFS3 with ab154543 at 1/500 dilution.
has not yet been referenced specifically in any publications.
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