Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Total protein in input
30 µg
Other product details
Concentration
2.5 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 50mM Tris-HCl(pH 8), 50mM KCl, 1mM EDTA,
Additional data
Additional Notes
IP buffer: 50mM Tris-HCl(pH 8), 50mM KCl, 1mM EDTA, 5% glycerol
Mock IP: 2.5 ´g/mg lysate goat serum.
Protein loading: Input - 10%, flow through (FT) - 10%, immunoprecipitate (IP) - 100%.
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提交于 Jan 07 2015