重组Anti-NDUFB8抗体[EPR15961] (ab192878)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15961] to NDUFB8
- Suitable for: ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-NDUFB8抗体[EPR15961]
参阅全部 NDUFB8 一抗 -
描述
兔单克隆抗体[EPR15961] to NDUFB8 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal liver, fetal heart and tonsil lysates; HeLa, C6, PC12, NIH 3T3 and RAW 264.7 cell lysates; Human kidney, Mouse brain and Rat kidney tissues; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15961 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab192878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/50.
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IP |
1/20.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
1/50. |
IP
1/20. |
WB
1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
序列相似性
Belongs to the complex I NDUFB8 subunit family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4714 Human
- Entrez Gene: 67264 Mouse
- Entrez Gene: 293991 Rat
- Omim: 602140 Human
- SwissProt: O95169 Human
- SwissProt: Q9D6J5 Mouse
- Unigene: 523215 Human
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别名
- ASHI antibody
- CI-ASHI antibody
- Complex I ASHI subunit antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed mouse skeletal muscle tissue permeabilized with 0.3% Triton X100 in PBS stained with ab192878 at 1/50 dilution. Secondary antibody was Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated at 1/3000 dilution. Samples were incubated with the primary antibody with 5% goat serum in 0.2% Triton X100 in PBS for 16 hours at 4°C. Blocking was done using 5% serum for 1 hour at 21°C. Heat mediated antigen retrieval with Tris/EDTA pH 9.0. ABC system used for signal amplification. Chromogenic reaction developed with DAB Substrate Kit (ab64238).Cell nuclei counterstained with Gill's hematoxylin I.
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All lanes : Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NDUFB8 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab192878 observed at 19 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab192878 was shown to specifically react with NDUFB8 in wild-type HAP1 cells as signal was lost in NDUFB8 knockout cells. Wild-type and NDUFB8 knockout samples were subjected to SDS-PAGE. Ab192878 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB8 antibody (red) with purified ab192878 at a dilution of 1/30. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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All lanes : Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/5000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human tonsil lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/20000 dilution + Human fetal heart lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-NDUFB8 antibody [EPR15961] (ab192878) at 1/5000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Western blot analysis of NDUFB8 immunoprecipitated from Human fetal heart lysate using ab192878 at 1/20 dilution. Lane 1: Human fetal liver lysate. Lane 2: PBS instead of Human fetal liver lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB8 with ab192878 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stained is DAPI (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (11)
ab192878 被引用在 11 文献中.
- Hao X et al. Disrupted mitochondrial homeostasis coupled with mitotic arrest generates antineoplastic oxidative stress. Oncogene 41:427-443 (2022). PubMed: 34773075
- Zhao M et al. Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy. Nat Commun 13:1757 (2022). PubMed: 35365608
- Zhu Z et al. Photobiomodulation promotes repair following spinal cord injury by restoring neuronal mitochondrial bioenergetics via AMPK/PGC-1α/TFAM pathway. Front Pharmacol 13:991421 (2022). PubMed: 36172183
- Xie L et al. Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex. Cell Rep 41:111498 (2022). PubMed: 36261001
- Bandara AB et al. Complex II subunit SDHD is critical for cell growth and metabolism, which can be partially restored with a synthetic ubiquinone analog. BMC Mol Cell Biol 22:35 (2021). PubMed: 34118887