Anti-NDUFB6抗体[21C11BC11] (ab110244)
Key features and details
- Mouse monoclonal [21C11BC11] to NDUFB6
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
概述
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产品名称
Anti-NDUFB6抗体[21C11BC11]
参阅全部 NDUFB6 一抗 -
描述
小鼠单克隆抗体[21C11BC11] to NDUFB6 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Full length protein. This information is considered to be commercially sensitive.
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阳性对照
- Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯化说明
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
21C11BC11 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110244于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
|
说明 |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa. |
靶标
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功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
序列相似性
Belongs to the complex I NDUFB6 subunit family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 327665 Cow
- Entrez Gene: 4712 Human
- Entrez Gene: 230075 Mouse
- Entrez Gene: 297990 Rat
- Omim: 603322 Human
- SwissProt: Q02367 Cow
- SwissProt: O95139 Human
- SwissProt: Q3UIU2 Mouse
see all -
别名
- B17 antibody
- CI antibody
- CI B17 antibody
see all
图片
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All lanes : Anti-NDUFB6 antibody [21C11BC11] (ab110244) at 0.5 µg/ml
Lane 1 : isolated mitochondria from Human heart at 5 µg
Lane 2 : isolated mitochondria from cow heart at 1 µg
Lane 3 : isolated mitochondria from rat heart at 10 µg
Lane 4 : isolated mitochondria from mouse heart at 10 µg
Predicted band size: 15 kDa -
Overlay histogram showing HepG2 cells stained with ab110244 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110244, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
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SDS download
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Datasheet download
文献 (35)
ab110244 被引用在 35 文献中.
- Zhu X et al. VDAC1 oligomerization may enhance DDP-induced hepatocyte apoptosis by exacerbating oxidative stress and mitochondrial DNA damage. FEBS Open Bio 12:516-522 (2022). PubMed: 34967508
- Al Khatib I et al. Functional characterization of two variants of mitochondrial topoisomerase TOP1MT that impact regulation of the mitochondrial genome. J Biol Chem 298:102420 (2022). PubMed: 36030054
- Pasák M et al. Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model. Biomolecules 12:N/A (2022). PubMed: 36291741
- Acoba MG et al. The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. Cell Rep 34:108869 (2021). PubMed: 33730581
- D'Angelo L et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep 35:109002 (2021). PubMed: 33882309