重组Anti-NDUFB11抗体[EPR15252] (ab183716)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15252] to NDUFB11
- Suitable for: WB, Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NDUFB11抗体[EPR15252]
参阅全部 NDUFB11 一抗 -
描述
兔单克隆抗体[EPR15252] to NDUFB11 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra), IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human heart lysate; HeLa, HepG2 and A431 cell lysates; Human kidney tissue; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15252 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183716于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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Flow Cyt (Intra) |
1/240.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
Flow Cyt (Intra)
1/240. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
NDUFB11 (NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11) is a component of the mitochondrial complex 1. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. Complex I transfers electrons from NADH to the respiratory chain via ubiquinone. -
细胞定位
Mitochondrion inner membrane. -
数据库链接
- Entrez Gene: 54539 Human
- Entrez Gene: 299310 Rat
- Omim: 300403 Human
- SwissProt: Q9NX14 Human
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别名
- CI ESSS antibody
- Complex I ESSS antibody
- complex I NP17.3 subunit antibody
see all
图片
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Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution + Human heart tissue lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Lanes 1-2 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/50000 dilution
Lane 3 : Anti-NDUFB11 antibody [EPR15252] (ab183716) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling NDUFB11 with ab183716 at a 1/250 dilution. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling NDUFB11 with ab183716 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab183716 被引用在 6 文献中.
- Timón-Gómez A et al. Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Cell Rep 31:107607 (2020). PubMed: 32375044
- Wilson BC et al. Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function. Nat Commun 11:5469 (2020). PubMed: 33122634
- Timón-Gómez A et al. Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis. STAR Protoc 1:N/A (2020). PubMed: 32995753
- Baertling F et al. NDUFA9 point mutations cause a variable mitochondrial complex I assembly defect. Clin Genet 93:111-118 (2018). WB ; Human . PubMed: 28671271
- Reinson K et al. Diverse phenotype in patients with complex I deficiency due to mutations in NDUFB11. Eur J Med Genet N/A:N/A (2018). PubMed: 30423443
- Stroud DA et al. Accessory subunits are integral for assembly and function of human mitochondrial complex I. Nature 538:123-126 (2016). PubMed: 27626371